Correction to “Single-Molecule Orientation Imaging Reveals the Nano-Architecture of Amyloid Fibrils Undergoing Growth and Decay”

In our original paper, there are errors in Figure 4 and the Supporting Information. Specifically, the color bars that describe the measured wobble Ω of Nile blue were erroneously inverted and should increase in intensity from purple to yellow with increasing wobble. These errors have been rectified in the revised Figure 4 below and the associated Supporting Information. We note that the errors are confined to the color bars describing wobble; no other data, discussions, or conclusions of our article are affected. The authors apologize for this mistake and any inconvenience caused by our oversight. Figure 4 SMOLM reveals architectural remodeling at the nanoscale. Top: SMLM images taken at 0 and 10 min. Bottom: SMOLM images of the subROI within white box (top). (Left) Orientation ϕ of each NB molecule, represented as a line segment aligned parallel to its orientation. (Right) Wobble Ω of each NB molecule. (a) Linear segment remodeling over 10 min. SubROIs (i–iii) (dashed yellow boxes) feature more disordered underlying assemblies on average than their neighboring subregions (iv,v) (green boxes). NB shows weaker binding to heterogeneous subROIs (i–iii) compared to neighbors in the core of the fiber (iv, v). Only small changes in segment shape are observed by SMLM (χ² = 0.129) in subROIs (i–iii). (b) Left edges of a linear segment remodel over 10 min. SubROIs (i–iii) (dashed yellow boxes) have more disordered underlying assemblies thanthe core of the fibril (iv–vi) (boxed in green). The fringes are also more structurally heterogeneous than their neighbors in the segment core.The growth of a disorganized offshoot in subROI (ii) (red dashed box at 10 min) is associated with only a small change in overall shape (χ² = 0.082). (c) A linear segment with a heterogeneous right section remodeling over 10 min, culminating in the formation of a rightward “bridgehead” structure (cyan box). The dynamic edges of the fibril in subROIs (iii, iv) (dashed yellow boxes) are more disordered and structurally heterogeneous than the segment core in subROIs (i, ii) (green boxes), with more dynamic changes in underlying assembly orientation and rigidityinthe fringes compared to the core. Color bars: (top, SMLM) NB localizations per 20 nm × 20 nm bin, (bottom left, SMOLM) NB orientation ϕ (rad), (bottom right, SMOLM) NB wobble Ω (sr). Scale bars: (top, SMLM) 200 nm, (bottom, SMOLM) 80 nm.