Corepression of ReIA and c-Rel inhibits immunoglobulin κ gene transcription and rearrangement in precursor B lymphocytes

David C. Scherer, Jeffrey A. Brockman, Heather H. Bendall, Guo Ming Zhang, Dean W. Ballard, Eugene M. Oltz

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Multiple members of the NF-κB/Rel protein family are induced during B cell differentiation and have been implicated in transcriptional activation of the immunoglobulin κ (Igκ) locus. Despite these findings, normal numbers of Igκ+ B lymphocytes are produced by mice bearing targeted mutations in individual NF-κB/Rel genes. In the present study, precursor B lymphocytes were engineered to express a trans-dominant form of IκBα that simultaneously impairs the c-Rel and RelA transactivating subunits of NF-κB. This dual block in NF-κB/Rel signaling led to potent inhibition of germline Igκ transcription and rearrangement, whereas recombinase activity was unaffected. These findings suggest that c-Rel and RelA serve compensatory functional roles in the developmental mechanisms that govern Igκ gene assembly.

Original languageEnglish
Pages (from-to)563-574
Number of pages12
JournalImmunity
Volume5
Issue number6
DOIs
StatePublished - Dec 1996

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