COQ4 is required for the oxidative decarboxylation of the C1 carbon of coenzyme Q in eukaryotic cells

Ludovic Pelosi, Laura Morbiato, Arthur Burgardt, Fiorella Tonello, Abigail K. Bartlett, Rachel M. Guerra, Katayoun Kazemzadeh Ferizhendi, Maria Andrea Desbats, Bérengère Rascalou, Marco Marchi, Luis Vázquez-Fonseca, Caterina Agosto, Giuseppe Zanotti, Morgane Roger-Margueritat, María Alcázar-Fabra, Laura García-Corzo, Ana Sánchez-Cuesta, Plácido Navas, Gloria Brea-Calvo, Eva TrevissonVolker F. Wendisch, David J. Pagliarini, Leonardo Salviati, Fabien Pierrel

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Coenzyme Q (CoQ) is a redox lipid that fulfills critical functions in cellular bioenergetics and homeostasis. CoQ is synthesized by a multi-step pathway that involves several COQ proteins. Two steps of the eukaryotic pathway, the decarboxylation and hydroxylation of position C1, have remained uncharacterized. Here, we provide evidence that these two reactions occur in a single oxidative decarboxylation step catalyzed by COQ4. We demonstrate that COQ4 complements an Escherichia coli strain deficient for C1 decarboxylation and hydroxylation and that COQ4 displays oxidative decarboxylation activity in the non-CoQ producer Corynebacterium glutamicum. Overall, our results substantiate that COQ4 contributes to CoQ biosynthesis, not only via its previously proposed structural role but also via the oxidative decarboxylation of CoQ precursors. These findings fill a major gap in the knowledge of eukaryotic CoQ biosynthesis and shed light on the pathophysiology of human primary CoQ deficiency due to COQ4 mutations.

Original languageEnglish
Pages (from-to)981-989.e7
JournalMolecular cell
Volume84
Issue number5
DOIs
StatePublished - Mar 7 2024

Keywords

  • COQ4
  • Corynebacterium
  • coenzyme Q
  • coenzyme Q biosynthesis
  • coenzyme Q deficiency
  • mitochondria
  • oxidative decarboxylation
  • respiratory chain

Fingerprint

Dive into the research topics of 'COQ4 is required for the oxidative decarboxylation of the C1 carbon of coenzyme Q in eukaryotic cells'. Together they form a unique fingerprint.

Cite this