Copper-64-labeled antibodies for PET imaging

C. J. Anderson; J. M. Connett; S. W. Schwarz; P. A. Rocque; Wu Guo Li Wu Guo; G. W. Philpott; K. R. Zinn; C. F. Meares; M. J. Welch

Copper-64-labeled antibodies for PET imaging

C. J. Anderson, J. M. Connett, S. W. Schwarz, P. A. Rocque, Wu Guo Li Wu Guo, G. W. Philpott, K. R. Zinn, C. F. Meares, M. J. Welch

Division of Research Imaging Facilities

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152 Scopus citations

Abstract

In the imaging of tumors using radiolabeled monoclonal antibodies, the use of PET gives increased sensitivity over conventional gamma camera imaging techniques. Copper-64, a positron-emitting radionuclide, has been labeled to 1A3, an anticolorectal carcinoma monoclonal antibody, and its fragments 1A3- F(ab')₂ utilizing the bifunctional chelate Br-benzyl-TETA. The ⁶⁴Cu- labeled intact 1A3 and 1A3-F(ab')₂ have been evaluated as potential imaging agents for PET. Biodistribution studies of ⁶⁴Cu-benzyl-TETA-1A3 and ⁶⁴Cu- benzyl-TETA-1A3-F(ab')₂ in tumor-bearing hamsters were compared with those of ¹¹¹In-BrΦHBED-1A3, ¹¹¹In-BrΦHBED-1A3-F(ab')₂ and ¹²⁶I-labeled intact 1A3 and 1A3-F(ab')₂. Tumor uptake of ⁶⁴Cu-labeled intact 1A3 and fragments in the hamster model was superior to both ¹¹¹In- and ¹²⁵I- labeled intact 1A3 and fragments. Human dosimetry data for ⁶⁴Cu- and ¹²³I-labeled 1A3 and 1A3-F(ab')₂ were calculated from biodistribution data in rats. High kidney uptake of ⁶⁴Cu-benzyl-TETA-1A3-F(ab')₂ precludes clinical study at this time; however, the data shows that ⁶⁴Cu-benzyl-TETA- 1A3 would be suitable for positron tomography imaging of colorectal cancer in patients.

Original language	English
Pages (from-to)	1685-1691
Number of pages	7
Journal	Journal of Nuclear Medicine
Volume	33
Issue number	9
State	Published - 1992

Cite this

@article{f5a07f07715d4327b315999e31fb27b2,

title = "Copper-64-labeled antibodies for PET imaging",

abstract = "In the imaging of tumors using radiolabeled monoclonal antibodies, the use of PET gives increased sensitivity over conventional gamma camera imaging techniques. Copper-64, a positron-emitting radionuclide, has been labeled to 1A3, an anticolorectal carcinoma monoclonal antibody, and its fragments 1A3- F(ab')2 utilizing the bifunctional chelate Br-benzyl-TETA. The 64Cu- labeled intact 1A3 and 1A3-F(ab')2 have been evaluated as potential imaging agents for PET. Biodistribution studies of 64Cu-benzyl-TETA-1A3 and 64Cu- benzyl-TETA-1A3-F(ab')2 in tumor-bearing hamsters were compared with those of 111In-BrΦHBED-1A3, 111In-BrΦHBED-1A3-F(ab')2 and 126I-labeled intact 1A3 and 1A3-F(ab')2. Tumor uptake of 64Cu-labeled intact 1A3 and fragments in the hamster model was superior to both 111In- and 125I- labeled intact 1A3 and fragments. Human dosimetry data for 64Cu- and 123I-labeled 1A3 and 1A3-F(ab')2 were calculated from biodistribution data in rats. High kidney uptake of 64Cu-benzyl-TETA-1A3-F(ab')2 precludes clinical study at this time; however, the data shows that 64Cu-benzyl-TETA- 1A3 would be suitable for positron tomography imaging of colorectal cancer in patients.",

author = "Anderson, {C. J.} and Connett, {J. M.} and Schwarz, {S. W.} and Rocque, {P. A.} and {Li Wu Guo}, {Wu Guo} and Philpott, {G. W.} and Zinn, {K. R.} and Meares, {C. F.} and Welch, {M. J.}",

year = "1992",

language = "English",

volume = "33",

pages = "1685--1691",

journal = "Journal of Nuclear Medicine",

issn = "0161-5505",

number = "9",

}

TY - JOUR

T1 - Copper-64-labeled antibodies for PET imaging

AU - Anderson, C. J.

AU - Connett, J. M.

AU - Schwarz, S. W.

AU - Rocque, P. A.

AU - Li Wu Guo, Wu Guo

AU - Philpott, G. W.

AU - Zinn, K. R.

AU - Meares, C. F.

AU - Welch, M. J.

PY - 1992

Y1 - 1992

N2 - In the imaging of tumors using radiolabeled monoclonal antibodies, the use of PET gives increased sensitivity over conventional gamma camera imaging techniques. Copper-64, a positron-emitting radionuclide, has been labeled to 1A3, an anticolorectal carcinoma monoclonal antibody, and its fragments 1A3- F(ab')2 utilizing the bifunctional chelate Br-benzyl-TETA. The 64Cu- labeled intact 1A3 and 1A3-F(ab')2 have been evaluated as potential imaging agents for PET. Biodistribution studies of 64Cu-benzyl-TETA-1A3 and 64Cu- benzyl-TETA-1A3-F(ab')2 in tumor-bearing hamsters were compared with those of 111In-BrΦHBED-1A3, 111In-BrΦHBED-1A3-F(ab')2 and 126I-labeled intact 1A3 and 1A3-F(ab')2. Tumor uptake of 64Cu-labeled intact 1A3 and fragments in the hamster model was superior to both 111In- and 125I- labeled intact 1A3 and fragments. Human dosimetry data for 64Cu- and 123I-labeled 1A3 and 1A3-F(ab')2 were calculated from biodistribution data in rats. High kidney uptake of 64Cu-benzyl-TETA-1A3-F(ab')2 precludes clinical study at this time; however, the data shows that 64Cu-benzyl-TETA- 1A3 would be suitable for positron tomography imaging of colorectal cancer in patients.

AB - In the imaging of tumors using radiolabeled monoclonal antibodies, the use of PET gives increased sensitivity over conventional gamma camera imaging techniques. Copper-64, a positron-emitting radionuclide, has been labeled to 1A3, an anticolorectal carcinoma monoclonal antibody, and its fragments 1A3- F(ab')2 utilizing the bifunctional chelate Br-benzyl-TETA. The 64Cu- labeled intact 1A3 and 1A3-F(ab')2 have been evaluated as potential imaging agents for PET. Biodistribution studies of 64Cu-benzyl-TETA-1A3 and 64Cu- benzyl-TETA-1A3-F(ab')2 in tumor-bearing hamsters were compared with those of 111In-BrΦHBED-1A3, 111In-BrΦHBED-1A3-F(ab')2 and 126I-labeled intact 1A3 and 1A3-F(ab')2. Tumor uptake of 64Cu-labeled intact 1A3 and fragments in the hamster model was superior to both 111In- and 125I- labeled intact 1A3 and fragments. Human dosimetry data for 64Cu- and 123I-labeled 1A3 and 1A3-F(ab')2 were calculated from biodistribution data in rats. High kidney uptake of 64Cu-benzyl-TETA-1A3-F(ab')2 precludes clinical study at this time; however, the data shows that 64Cu-benzyl-TETA- 1A3 would be suitable for positron tomography imaging of colorectal cancer in patients.

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M3 - Article

C2 - 1517844

AN - SCOPUS:0026778983

SN - 0161-5505

VL - 33

SP - 1685

EP - 1691

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

IS - 9

ER -

Copper-64-labeled antibodies for PET imaging

Abstract

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