Convulsant γ-butyrolactones block GABA currents in cultured chick spinal cord neurons

Charles F. Zorumski; Jay Yang; Keith Baker; Douglas F. Covey; David B. Clifford

doi:10.1016/0006-8993(89)90352-1

Convulsant γ-butyrolactones block GABA currents in cultured chick spinal cord neurons

Charles F. Zorumski, Jay Yang, Keith Baker, Douglas F. Covey, David B. Clifford

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The γ-aminobutyric acid (GABA) inhibiting properties of the β-substituted γ-butyrolactone convulsant, β-isopropyl-γ-butyrolactone (βIPGBL), were studied using gigaseal recording techniques in cultured chick spinal cord neurons. βIPGBL produced a dose-dependent inhibition of GABA currents with half maximal effect at 92 μM. The effects of βIPGBL were immediate and completely reversible within minutes after exposure. The inhibition by βIPGBL showed mixed competitive and non-competitive features with little voltage-dependence. βIPGBL did not alter the GABA reversal potential nor the degree of GABA desensitization. At a single-channel level, βIPGBL markedly diminished the opening of GABA channels and decreased the mean channel open time by 30-40% without affecting the amplitude of the single-channel current.

Original language	English
Pages (from-to)	102-110
Number of pages	9
Journal	Brain Research
Volume	484
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(89)90352-1
State	Published - Apr 10 1989

Keywords

GABA
Patch-clamp
Picrotoxinin
Spinal cord neuron
Voltage-clamp
t-Butylbicyclophosphorothionate (TBPS)
γ-Aminobutyric acid

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10.1016/0006-8993(89)90352-1

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@article{917197e61673411dadd9c15b99863a8e,

title = "Convulsant γ-butyrolactones block GABA currents in cultured chick spinal cord neurons",

abstract = "The γ-aminobutyric acid (GABA) inhibiting properties of the β-substituted γ-butyrolactone convulsant, β-isopropyl-γ-butyrolactone (βIPGBL), were studied using gigaseal recording techniques in cultured chick spinal cord neurons. βIPGBL produced a dose-dependent inhibition of GABA currents with half maximal effect at 92 μM. The effects of βIPGBL were immediate and completely reversible within minutes after exposure. The inhibition by βIPGBL showed mixed competitive and non-competitive features with little voltage-dependence. βIPGBL did not alter the GABA reversal potential nor the degree of GABA desensitization. At a single-channel level, βIPGBL markedly diminished the opening of GABA channels and decreased the mean channel open time by 30-40% without affecting the amplitude of the single-channel current.",

keywords = "GABA, Patch-clamp, Picrotoxinin, Spinal cord neuron, Voltage-clamp, t-Butylbicyclophosphorothionate (TBPS), γ-Aminobutyric acid",

author = "Zorumski, {Charles F.} and Jay Yang and Keith Baker and Covey, {Douglas F.} and Clifford, {David B.}",

year = "1989",

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doi = "10.1016/0006-8993(89)90352-1",

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T1 - Convulsant γ-butyrolactones block GABA currents in cultured chick spinal cord neurons

AU - Zorumski, Charles F.

AU - Yang, Jay

AU - Baker, Keith

AU - Covey, Douglas F.

AU - Clifford, David B.

PY - 1989/4/10

Y1 - 1989/4/10

N2 - The γ-aminobutyric acid (GABA) inhibiting properties of the β-substituted γ-butyrolactone convulsant, β-isopropyl-γ-butyrolactone (βIPGBL), were studied using gigaseal recording techniques in cultured chick spinal cord neurons. βIPGBL produced a dose-dependent inhibition of GABA currents with half maximal effect at 92 μM. The effects of βIPGBL were immediate and completely reversible within minutes after exposure. The inhibition by βIPGBL showed mixed competitive and non-competitive features with little voltage-dependence. βIPGBL did not alter the GABA reversal potential nor the degree of GABA desensitization. At a single-channel level, βIPGBL markedly diminished the opening of GABA channels and decreased the mean channel open time by 30-40% without affecting the amplitude of the single-channel current.

AB - The γ-aminobutyric acid (GABA) inhibiting properties of the β-substituted γ-butyrolactone convulsant, β-isopropyl-γ-butyrolactone (βIPGBL), were studied using gigaseal recording techniques in cultured chick spinal cord neurons. βIPGBL produced a dose-dependent inhibition of GABA currents with half maximal effect at 92 μM. The effects of βIPGBL were immediate and completely reversible within minutes after exposure. The inhibition by βIPGBL showed mixed competitive and non-competitive features with little voltage-dependence. βIPGBL did not alter the GABA reversal potential nor the degree of GABA desensitization. At a single-channel level, βIPGBL markedly diminished the opening of GABA channels and decreased the mean channel open time by 30-40% without affecting the amplitude of the single-channel current.

KW - GABA

KW - Patch-clamp

KW - Picrotoxinin

KW - Spinal cord neuron

KW - Voltage-clamp

KW - t-Butylbicyclophosphorothionate (TBPS)

KW - γ-Aminobutyric acid

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DO - 10.1016/0006-8993(89)90352-1

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AN - SCOPUS:0024588122

SN - 0006-8993

VL - 484

SP - 102

EP - 110

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Convulsant γ-butyrolactones block GABA currents in cultured chick spinal cord neurons

Abstract

Keywords

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