TY - JOUR
T1 - Converting heterodimeric gonadotropins to genetically linked single chains
T2 - New approaches to structure activity relationships and analogue design
AU - Ben-Menahem, David
AU - Boime, Irving
N1 - Funding Information:
The authors thank Drs. Mesut Muyan, David Ornitz, and Ted Hansen for helpful suggestions regarding the manuscript. The authors are grateful for the excellent preparative assistance of Susan Cames. David Ben-Met&rem is supported by a grant from the Lalor Foundation. This work was supported by grants from the Organon Company and the National Institutes of Health (contract no. NOl-HD92922). These studies were done in collaboration with Dr. Aaron J.W. Hsueh, Department of Gynecology/Obstetrics, Division of Reproductive Biology, Stanford University Medical Center.
PY - 1996/4
Y1 - 1996/4
N2 - One of the distinguishing features of the gonadotropin and thyrotropin hormone family is their heterodimeric structure; the subunits combine early in the secretory pathway and only the dimers are capable of binding to receptors. Therefore, assembly is rate limiting in the production of functional heterodimers, a problem encountered when removing the carbohydrates from one or both subunits as discussed in this review. If the heterodimers can be expressed as single chains, this might avoid mutagenesis- induced defects in secretion and combination of individual subunits for structure-function studies and analogue design. Here we discuss the feasibility of this approach for such problems.
AB - One of the distinguishing features of the gonadotropin and thyrotropin hormone family is their heterodimeric structure; the subunits combine early in the secretory pathway and only the dimers are capable of binding to receptors. Therefore, assembly is rate limiting in the production of functional heterodimers, a problem encountered when removing the carbohydrates from one or both subunits as discussed in this review. If the heterodimers can be expressed as single chains, this might avoid mutagenesis- induced defects in secretion and combination of individual subunits for structure-function studies and analogue design. Here we discuss the feasibility of this approach for such problems.
UR - http://www.scopus.com/inward/record.url?scp=0029975957&partnerID=8YFLogxK
U2 - 10.1016/1043-2760(96)88667-X
DO - 10.1016/1043-2760(96)88667-X
M3 - Short survey
C2 - 18406733
AN - SCOPUS:0029975957
SN - 1043-2760
VL - 7
SP - 100
EP - 105
JO - Trends in Endocrinology and Metabolism
JF - Trends in Endocrinology and Metabolism
IS - 3
ER -