Conversion of monocytes to cells capable of anchorage-independent growth in vitro

H. S. Lin, S. Gordon, D. M. Chen, M. Kurtz

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We investigated the time-course involved in the conversion of mouse blood monocytes in vitro to cells capable of anchorage-independent growth. Two criteria were used to define when monocytes were fully converted to cells similar to mononuclear phagocytes present in inflammatory exudate, such as thioglycollate medium (TM)-elicited peritoneal exudate. They were the production of high levels of plasminogen activators and an ability to undergo anchorage-independent growth. Resident peritoneal macrophages were used as controls and for comparison. Our studies indicated that monocytes, but not resident peritoneal macrophages, could be converted to cells similar to TM-elicited mononuclear phagocytes after 2 d in culture.

    Original languageEnglish
    Pages (from-to)488-493
    Number of pages6
    JournalJournal of Experimental Medicine
    Volume153
    Issue number2
    DOIs
    StatePublished - 1981

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