Convergence and extension movements affect dynamic notochord-somite interactions essential for zebrafish slow muscle morphogenesis

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15 Scopus citations

Abstract

During vertebrate gastrulation, convergence and extension (C&E) movements shape and position the somites that form the fast and slow muscles. In zebrafish knypek;trilobite non-canonical Wnt mutants, defective C&E movements cause misshapen somites and reduction of slow muscle precursors, the adaxial cells. Here, we demonstrate essential roles of C&E in slow muscle morphogenesis. During segmentation, the adaxial cells change shapes and migrate laterally to form slow muscles at the myotome surface. Using confocal imaging techniques, we show that the adaxial cells undergo three-step shape changes, including dorsoventral elongation, anterior-ward rotation, and anteroposterior elongation. The adaxial cells in knypek;trilobite double mutants maintain prolonged contact with the notochord and fail to rotate anteriorly. Such a defect was suppressed by physical removal of their notochord or by introducing wild-type notochord cells into the mutant. We propose that in the double mutants, impaired C&E movements disrupt notochord development, which impedes the adaxial cell shape changes.

Original languageEnglish
Pages (from-to)2742-2756
Number of pages15
JournalDevelopmental Dynamics
Volume236
Issue number10
DOIs
StatePublished - Oct 2007

Keywords

  • Adaxial cell
  • Fibronectin
  • Gastrulation
  • Knypek
  • Non-canonical Wnt
  • Somitic boundary
  • Trilobite

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