Control of the proliferation versus meiotic development decision in the C. elegans germline through regulation of GLD-1 protein accumulation

Dave Hansen, Laura Wilson-Berry, Thanh Dang, Tim Schedl

Research output: Contribution to journalArticle

128 Scopus citations

Abstract

Maintenance of the stem cell population in the C. elegans germline requires GLP-1/Notch signaling. We show that this signaling inhibits the accumulation of the RNA binding protein GLD-1. In a genetic screen to identify other genes involved in regulating GLD-1 activity, we identified mutations in the nos-3 gene, the protein product of which is similar to the Drosophila translational regulator Nanos. Our data demonstrate that nos-3 promotes GLD-1 accumulation redundantly with gld-2, and that nos-3 functions genetically downstream or parallel to fbf, an inhibitor of GLD-1 translation. We show that the GLD-1 accumulation pattern is important in controlling the proliferation versus meiotic development decision, with low GLD-1 levels allowing proliferation and increased levels promoting meiotic entry.

Original languageEnglish
Pages (from-to)93-104
Number of pages12
JournalDevelopment
Volume131
Issue number1
DOIs
StatePublished - Jan 1 2004

Keywords

  • Germline development
  • Meiotic entry
  • Notch signaling
  • Proliferation
  • Stem cells
  • Tumor
  • gld-1
  • glp-1
  • nos-3

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