Control of leaf morphogenesis by microRNAs

Javier F. Palatnik; Edwards Allen; Xuelin Wu; Carla Schommer; Rebecca Schwab; James C. Carrington; Detlef Weigel

doi:10.1038/nature01958

Control of leaf morphogenesis by microRNAs

Javier F. Palatnik
, Edwards Allen
, Xuelin Wu
, Carla Schommer
, Rebecca Schwab
, James C. Carrington
, Detlef Weigel

Research output: Contribution to journal › Article › peer-review

1585 Scopus citations

Abstract

Plants with altered microRNA metabolism have pleiotropic developmental defects, but direct evidence for microRNAs regulating specific aspects of plant morphogenesis has been lacking. In a genetic screen, we identified the JAW locus, which produces a microRNA that can guide messenger RNA cleavage of several TCP genes controlling leaf development. MicroRNA-guided cleavage of TCP4 mRNA is necessary to prevent aberrant activity of the TCP4 gene expressed from its native promoter. In addition, overexpression of wild-type and microRNA-resistant TCP variants demonstrates that mRNA cleavage is largely sufficient to restrict TCP function to its normal domain of activity. TCP genes with microRNA target sequences are found in a wide range of species, indicating that microRNA-mediated control of leaf morphogenesis is conserved in plants with very different leaf forms.

Original language	English
Pages (from-to)	257-263
Number of pages	7
Journal	Nature
Volume	425
Issue number	6955
DOIs	https://doi.org/10.1038/nature01958
State	Published - Sep 18 2003

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@article{3461e831c03e4a2981b951b7090504b6,

title = "Control of leaf morphogenesis by microRNAs",

abstract = "Plants with altered microRNA metabolism have pleiotropic developmental defects, but direct evidence for microRNAs regulating specific aspects of plant morphogenesis has been lacking. In a genetic screen, we identified the JAW locus, which produces a microRNA that can guide messenger RNA cleavage of several TCP genes controlling leaf development. MicroRNA-guided cleavage of TCP4 mRNA is necessary to prevent aberrant activity of the TCP4 gene expressed from its native promoter. In addition, overexpression of wild-type and microRNA-resistant TCP variants demonstrates that mRNA cleavage is largely sufficient to restrict TCP function to its normal domain of activity. TCP genes with microRNA target sequences are found in a wide range of species, indicating that microRNA-mediated control of leaf morphogenesis is conserved in plants with very different leaf forms.",

author = "Palatnik, \{Javier F.\} and Edwards Allen and Xuelin Wu and Carla Schommer and Rebecca Schwab and Carrington, \{James C.\} and Detlef Weigel",

note = "Funding Information: Acknowledgements We thank I. Puga-Gonzalez for assistance and V. Ambros, S. Balasubramanian, J. Chory, M.-C. Kim, J. Lohmann, Y. Kobayashi and J. Spatafora for advice and discussion. This work was supported by fellowships from CONICET and Human Frontier Science Program Organization to J.F.P., from Life Sciences Research Foundation/US Department of Energy to X.W.; by grants from NSF and NIH to J.C.C. and NIH to D.W.; and by the Max Planck Society. D.W. is a Director of the Max Planck Institute.",

year = "2003",

month = sep,

day = "18",

doi = "10.1038/nature01958",

language = "English",

volume = "425",

pages = "257--263",

journal = "Nature",

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TY - JOUR

T1 - Control of leaf morphogenesis by microRNAs

AU - Palatnik, Javier F.

AU - Allen, Edwards

AU - Wu, Xuelin

AU - Schommer, Carla

AU - Schwab, Rebecca

AU - Carrington, James C.

AU - Weigel, Detlef

N1 - Funding Information: Acknowledgements We thank I. Puga-Gonzalez for assistance and V. Ambros, S. Balasubramanian, J. Chory, M.-C. Kim, J. Lohmann, Y. Kobayashi and J. Spatafora for advice and discussion. This work was supported by fellowships from CONICET and Human Frontier Science Program Organization to J.F.P., from Life Sciences Research Foundation/US Department of Energy to X.W.; by grants from NSF and NIH to J.C.C. and NIH to D.W.; and by the Max Planck Society. D.W. is a Director of the Max Planck Institute.

PY - 2003/9/18

Y1 - 2003/9/18

N2 - Plants with altered microRNA metabolism have pleiotropic developmental defects, but direct evidence for microRNAs regulating specific aspects of plant morphogenesis has been lacking. In a genetic screen, we identified the JAW locus, which produces a microRNA that can guide messenger RNA cleavage of several TCP genes controlling leaf development. MicroRNA-guided cleavage of TCP4 mRNA is necessary to prevent aberrant activity of the TCP4 gene expressed from its native promoter. In addition, overexpression of wild-type and microRNA-resistant TCP variants demonstrates that mRNA cleavage is largely sufficient to restrict TCP function to its normal domain of activity. TCP genes with microRNA target sequences are found in a wide range of species, indicating that microRNA-mediated control of leaf morphogenesis is conserved in plants with very different leaf forms.

AB - Plants with altered microRNA metabolism have pleiotropic developmental defects, but direct evidence for microRNAs regulating specific aspects of plant morphogenesis has been lacking. In a genetic screen, we identified the JAW locus, which produces a microRNA that can guide messenger RNA cleavage of several TCP genes controlling leaf development. MicroRNA-guided cleavage of TCP4 mRNA is necessary to prevent aberrant activity of the TCP4 gene expressed from its native promoter. In addition, overexpression of wild-type and microRNA-resistant TCP variants demonstrates that mRNA cleavage is largely sufficient to restrict TCP function to its normal domain of activity. TCP genes with microRNA target sequences are found in a wide range of species, indicating that microRNA-mediated control of leaf morphogenesis is conserved in plants with very different leaf forms.

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U2 - 10.1038/nature01958

DO - 10.1038/nature01958

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AN - SCOPUS:0141493493

SN - 0028-0836

VL - 425

SP - 257

EP - 263

JO - Nature

JF - Nature

IS - 6955

ER -

Control of leaf morphogenesis by microRNAs

Abstract

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