Control of globin gene expression by steroid hormones in differentiating friend leukemia cells

Shyh Ching Lo; Rebecca Aft; Jeffrey Ross; Gerald C. Mueller

doi:10.1016/0092-8674(78)90014-4

Control of globin gene expression by steroid hormones in differentiating friend leukemia cells

Shyh Ching Lo, Rebecca Aft, Jeffrey Ross, Gerald C. Mueller

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Low levels of dexamethasone and related gluco-corticoid hormones suppress the expression of globin genes during the DMSO-induced differentiation of Friend leukemia cells. In this response, the glucocorticoids appear to act at both the transcriptional and post-transcriptional levels in that 10^-8 M dexamethasone prevents the accumulation of both globin mRNA and globin protein, whereas 10^-9 M dexamethasone allows the accumulation of normal levels of hybridizable globin mRNA but prevents the accumulation of globin protein. This suppressive action of dexamethasone is more effective with DMSO as the inducer of globin gene expression than with hemin as the inducer. In contrast to the situation with glucocorticoids, certain sex steroids (etiocholanolone, testosterone and estradiol) facilitate the expression of globin genes in DMSO-treated Friend leukemia cells. The modulation of globin gene expression by steroid hormones is achieved in DMSO-treated cells without altering the growth and morphological changes which characteristically attend the differentiation of these cells.

Original language	English
Pages (from-to)	447-453
Number of pages	7
Journal	Cell
Volume	15
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(78)90014-4
State	Published - Oct 1978

Access to Document

10.1016/0092-8674(78)90014-4

Cite this

@article{9a1ebb69ad464973959c9a876175b8c5,

title = "Control of globin gene expression by steroid hormones in differentiating friend leukemia cells",

abstract = "Low levels of dexamethasone and related gluco-corticoid hormones suppress the expression of globin genes during the DMSO-induced differentiation of Friend leukemia cells. In this response, the glucocorticoids appear to act at both the transcriptional and post-transcriptional levels in that 10-8 M dexamethasone prevents the accumulation of both globin mRNA and globin protein, whereas 10-9 M dexamethasone allows the accumulation of normal levels of hybridizable globin mRNA but prevents the accumulation of globin protein. This suppressive action of dexamethasone is more effective with DMSO as the inducer of globin gene expression than with hemin as the inducer. In contrast to the situation with glucocorticoids, certain sex steroids (etiocholanolone, testosterone and estradiol) facilitate the expression of globin genes in DMSO-treated Friend leukemia cells. The modulation of globin gene expression by steroid hormones is achieved in DMSO-treated cells without altering the growth and morphological changes which characteristically attend the differentiation of these cells.",

author = "Lo, {Shyh Ching} and Rebecca Aft and Jeffrey Ross and Mueller, {Gerald C.}",

note = "Funding Information: This study was supported by USPHS grants. G.C.M. is the recipient of a USPHS Research Career Award. The authors thank Ms. Mary LeMahieu for her help with the preparation of the manuscript. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.",

year = "1978",

month = oct,

doi = "10.1016/0092-8674(78)90014-4",

language = "English",

volume = "15",

pages = "447--453",

journal = "Cell",

issn = "0092-8674",

number = "2",

}

TY - JOUR

T1 - Control of globin gene expression by steroid hormones in differentiating friend leukemia cells

AU - Lo, Shyh Ching

AU - Aft, Rebecca

AU - Ross, Jeffrey

AU - Mueller, Gerald C.

N1 - Funding Information: This study was supported by USPHS grants. G.C.M. is the recipient of a USPHS Research Career Award. The authors thank Ms. Mary LeMahieu for her help with the preparation of the manuscript. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

PY - 1978/10

Y1 - 1978/10

N2 - Low levels of dexamethasone and related gluco-corticoid hormones suppress the expression of globin genes during the DMSO-induced differentiation of Friend leukemia cells. In this response, the glucocorticoids appear to act at both the transcriptional and post-transcriptional levels in that 10-8 M dexamethasone prevents the accumulation of both globin mRNA and globin protein, whereas 10-9 M dexamethasone allows the accumulation of normal levels of hybridizable globin mRNA but prevents the accumulation of globin protein. This suppressive action of dexamethasone is more effective with DMSO as the inducer of globin gene expression than with hemin as the inducer. In contrast to the situation with glucocorticoids, certain sex steroids (etiocholanolone, testosterone and estradiol) facilitate the expression of globin genes in DMSO-treated Friend leukemia cells. The modulation of globin gene expression by steroid hormones is achieved in DMSO-treated cells without altering the growth and morphological changes which characteristically attend the differentiation of these cells.

AB - Low levels of dexamethasone and related gluco-corticoid hormones suppress the expression of globin genes during the DMSO-induced differentiation of Friend leukemia cells. In this response, the glucocorticoids appear to act at both the transcriptional and post-transcriptional levels in that 10-8 M dexamethasone prevents the accumulation of both globin mRNA and globin protein, whereas 10-9 M dexamethasone allows the accumulation of normal levels of hybridizable globin mRNA but prevents the accumulation of globin protein. This suppressive action of dexamethasone is more effective with DMSO as the inducer of globin gene expression than with hemin as the inducer. In contrast to the situation with glucocorticoids, certain sex steroids (etiocholanolone, testosterone and estradiol) facilitate the expression of globin genes in DMSO-treated Friend leukemia cells. The modulation of globin gene expression by steroid hormones is achieved in DMSO-treated cells without altering the growth and morphological changes which characteristically attend the differentiation of these cells.

UR - http://www.scopus.com/inward/record.url?scp=0018114232&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(78)90014-4

DO - 10.1016/0092-8674(78)90014-4

M3 - Article

C2 - 281261

AN - SCOPUS:0018114232

SN - 0092-8674

VL - 15

SP - 447

EP - 453

JO - Cell

JF - Cell

IS - 2

ER -

Control of globin gene expression by steroid hormones in differentiating friend leukemia cells

Abstract

Access to Document

Other files and links

Fingerprint

Cite this