TY - JOUR
T1 - Control of actin assembly and disassembly at filament ends
AU - Cooper, John A.
AU - Schafer, Dorothy A.
N1 - Funding Information:
For critical discussions and unpublished work, the authors are grateful to Velia Fowler, Pekka Lappaleinen, Laura Machesky, Marie-France Carlier, Amy McGough, Laurent Blanchoin, Alan Weeds, Michael Way and Dyche Mullins. The writing of this article was supported by National Institutes of Health grant 38542.
PY - 2000/2/1
Y1 - 2000/2/1
N2 - The most important discovery in the field is that the Arp2/3 complex nucleates assembly of actin filaments with free barbed ends. Arp2/3 also binds the sides of actin filaments to create a branched network. Arp2/3's nucleation activity is stimulated by WASP family proteins, some of which mediate signaling from small G-proteins. Listeria movement caused by actin polymerization can be reconstituted in vitro using purified proteins: Arp2/3 complex, capping protein, actin depolymerizing factor/cofilin, and actin. actin depolymerizing factor/cofilin increases the rate at which actin subunits leave pointed ends, and capping protein caps barbed ends.
AB - The most important discovery in the field is that the Arp2/3 complex nucleates assembly of actin filaments with free barbed ends. Arp2/3 also binds the sides of actin filaments to create a branched network. Arp2/3's nucleation activity is stimulated by WASP family proteins, some of which mediate signaling from small G-proteins. Listeria movement caused by actin polymerization can be reconstituted in vitro using purified proteins: Arp2/3 complex, capping protein, actin depolymerizing factor/cofilin, and actin. actin depolymerizing factor/cofilin increases the rate at which actin subunits leave pointed ends, and capping protein caps barbed ends.
UR - http://www.scopus.com/inward/record.url?scp=0033965314&partnerID=8YFLogxK
U2 - 10.1016/S0955-0674(99)00062-9
DO - 10.1016/S0955-0674(99)00062-9
M3 - Review article
C2 - 10679358
AN - SCOPUS:0033965314
SN - 0955-0674
VL - 12
SP - 97
EP - 103
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
IS - 1
ER -