Continuous real-time monitoring of metabolic parameters in growing bacterial cultures

Jack H. Ladenson; M. Huebner; J. Joseph Marr

doi:10.1016/0003-2697(75)90188-8

Continuous real-time monitoring of metabolic parameters in growing bacterial cultures

Jack H. Ladenson, M. Huebner, J. Joseph Marr

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Abstract

Continuous monitoring of a bacterial culture for pH, growth, CO₂, and NH₃ has been accomplished by means of in situ probes. Changes in the metabolic parameters of Proteus cultures generally occurred about an hour before changes in growth were observed. The time of maximum CO₂ production preceded that of NH₃ elaboration by this organism; however, the sequence was reversed when urea was added to the medium. This type of in situ monitoring system has great potential for the study of the metabolism of growing organisms as well as for the early detection of growth in liquid culture.

Original language	English
Pages (from-to)	56-67
Number of pages	12
Journal	Analytical Biochemistry
Volume	63
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(75)90188-8
State	Published - Jan 1975

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abstract = "Continuous monitoring of a bacterial culture for pH, growth, CO2, and NH3 has been accomplished by means of in situ probes. Changes in the metabolic parameters of Proteus cultures generally occurred about an hour before changes in growth were observed. The time of maximum CO2 production preceded that of NH3 elaboration by this organism; however, the sequence was reversed when urea was added to the medium. This type of in situ monitoring system has great potential for the study of the metabolism of growing organisms as well as for the early detection of growth in liquid culture.",

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N2 - Continuous monitoring of a bacterial culture for pH, growth, CO2, and NH3 has been accomplished by means of in situ probes. Changes in the metabolic parameters of Proteus cultures generally occurred about an hour before changes in growth were observed. The time of maximum CO2 production preceded that of NH3 elaboration by this organism; however, the sequence was reversed when urea was added to the medium. This type of in situ monitoring system has great potential for the study of the metabolism of growing organisms as well as for the early detection of growth in liquid culture.

AB - Continuous monitoring of a bacterial culture for pH, growth, CO2, and NH3 has been accomplished by means of in situ probes. Changes in the metabolic parameters of Proteus cultures generally occurred about an hour before changes in growth were observed. The time of maximum CO2 production preceded that of NH3 elaboration by this organism; however, the sequence was reversed when urea was added to the medium. This type of in situ monitoring system has great potential for the study of the metabolism of growing organisms as well as for the early detection of growth in liquid culture.

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Continuous real-time monitoring of metabolic parameters in growing bacterial cultures

Abstract

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