Abstract
Continuous monitoring of a bacterial culture for pH, growth, CO2, and NH3 has been accomplished by means of in situ probes. Changes in the metabolic parameters of Proteus cultures generally occurred about an hour before changes in growth were observed. The time of maximum CO2 production preceded that of NH3 elaboration by this organism; however, the sequence was reversed when urea was added to the medium. This type of in situ monitoring system has great potential for the study of the metabolism of growing organisms as well as for the early detection of growth in liquid culture.
Original language | English |
---|---|
Pages (from-to) | 56-67 |
Number of pages | 12 |
Journal | Analytical Biochemistry |
Volume | 63 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1975 |