Construction of small RNA cDNA libraries for deep sequencing

Cheng Lu; Blake C. Meyers; Pamela J. Green

doi:10.1016/j.ymeth.2007.05.002

Construction of small RNA cDNA libraries for deep sequencing

Cheng Lu
, Blake C. Meyers
, Pamela J. Green

Research output: Contribution to journal › Article › peer-review

207 Scopus citations

Abstract

Small RNAs (21-24 nucleotides) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) are potent regulators of gene expression in both plants and animals. Several hundred genes encoding miRNAs and thousands of siRNAs have been experimentally identified by cloning approaches. New sequencing technologies facilitate the identification of these molecules and provide global quantitative expression data in a given biological sample. Here, we describe the methods used in our laboratory to construct small RNA cDNA libraries for high-throughput sequencing using technologies such as MPSS, 454 or SBS.

Original language	English
Pages (from-to)	110-117
Number of pages	8
Journal	Methods
Volume	43
Issue number	2
DOIs	https://doi.org/10.1016/j.ymeth.2007.05.002
State	Published - Oct 2007

Keywords

454
High-throughput sequencing
miRNAs
siRNAs
Small RNAs

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10.1016/j.ymeth.2007.05.002

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title = "Construction of small RNA cDNA libraries for deep sequencing",

abstract = "Small RNAs (21-24 nucleotides) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) are potent regulators of gene expression in both plants and animals. Several hundred genes encoding miRNAs and thousands of siRNAs have been experimentally identified by cloning approaches. New sequencing technologies facilitate the identification of these molecules and provide global quantitative expression data in a given biological sample. Here, we describe the methods used in our laboratory to construct small RNA cDNA libraries for high-throughput sequencing using technologies such as MPSS, 454 or SBS.",

keywords = "454, High-throughput sequencing, miRNAs, siRNAs, Small RNAs",

author = "Cheng Lu and Meyers, \{Blake C.\} and Green, \{Pamela J.\}",

note = "Funding Information: We thank S. Luo and C.D. Haudenschild for technical advice and assistance; M. German and M. Accerbi for comments on the manuscript. This work was supported primarily by NSF Grants 0439186 and 0548569 (P.J.G. and B.C.M.), with additional support provided by DOE DE-FG02-04ER15541 (P.J.G.).",

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AU - Meyers, Blake C.

AU - Green, Pamela J.

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PY - 2007/10

Y1 - 2007/10

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AB - Small RNAs (21-24 nucleotides) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) are potent regulators of gene expression in both plants and animals. Several hundred genes encoding miRNAs and thousands of siRNAs have been experimentally identified by cloning approaches. New sequencing technologies facilitate the identification of these molecules and provide global quantitative expression data in a given biological sample. Here, we describe the methods used in our laboratory to construct small RNA cDNA libraries for high-throughput sequencing using technologies such as MPSS, 454 or SBS.

KW - 454

KW - High-throughput sequencing

KW - miRNAs

KW - siRNAs

KW - Small RNAs

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SN - 1046-2023

VL - 43

SP - 110

EP - 117

JO - Methods

JF - Methods

IS - 2

ER -

Construction of small RNA cDNA libraries for deep sequencing

Abstract

Keywords

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