TY - JOUR
T1 - Conserved roles for Slit and Robo proteins in midline commissural axon guidance
AU - Long, Hua
AU - Sabatier, Christelle
AU - Ma, Le
AU - Plump, Andrew
AU - Yuan, Wenlin
AU - Ornitz, David M.
AU - Tamada, Atsushi
AU - Murakami, Fujio
AU - Goodman, Corey S.
AU - Tessier-Lavigne, Marc
N1 - Funding Information:
We thank A. Yaron, F. Charron, F. Wang, X. Lu, R. Friedel, M. Huse, and other members of the Tessier-Lavigne lab for helpful discussions and for sharing expertise and reagents, and H. Lin and S. Faynboym for technical assistance. H.L. was supported by a predoctoral fellowship from the Howard Hughes Medical Institute, C.S. by a predoctoral fellowship from the National Science Foundation, L.M. by a postdoctoral fellowship from the Damon Runyon Cancer Foundation, D.M.O. by NIH grant DCO4289, F.M. by SORST, Japan Science and Technology Corporation, and M.T.-L. and C.S.G. by the Howard Hughes Medical Institute. C.S.G. and M.T.-L. disclose competing financial interests (see Supplemental Data at http://www.neuron.org/cgi/content/full/42/2/213/DC1 ).
PY - 2004/4/22
Y1 - 2004/4/22
N2 - In Drosophila, Slit at the midline activates Robo receptors on commissural axons, thereby repelling them out of the midline into distinct longitudinal tracts on the contralateral side of the central nervous system. In the vertebrate spinal cord, Robo1 and Robo2 are expressed by commissural neurons, whereas all three Slit homologs are expressed at the ventral midline. Previous analysis of Slit1;Slit2 double mutant spinal cords failed to reveal a defect in commissural axon guidance. We report here that when all six Slit alleles are removed, many commissural axons fail to leave the midline, while others recross it. In addition, Robo1 and Robo2 single mutants show guidance defects that reveal a role for these two receptors in guiding commissural axons to different positions within the ventral and lateral funiculi. These results demonstrate a key role for Slit/Robo signaling in midline commissural axon guidance in vertebrates.
AB - In Drosophila, Slit at the midline activates Robo receptors on commissural axons, thereby repelling them out of the midline into distinct longitudinal tracts on the contralateral side of the central nervous system. In the vertebrate spinal cord, Robo1 and Robo2 are expressed by commissural neurons, whereas all three Slit homologs are expressed at the ventral midline. Previous analysis of Slit1;Slit2 double mutant spinal cords failed to reveal a defect in commissural axon guidance. We report here that when all six Slit alleles are removed, many commissural axons fail to leave the midline, while others recross it. In addition, Robo1 and Robo2 single mutants show guidance defects that reveal a role for these two receptors in guiding commissural axons to different positions within the ventral and lateral funiculi. These results demonstrate a key role for Slit/Robo signaling in midline commissural axon guidance in vertebrates.
UR - http://www.scopus.com/inward/record.url?scp=3242704800&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(04)00179-5
DO - 10.1016/S0896-6273(04)00179-5
M3 - Article
C2 - 15091338
AN - SCOPUS:3242704800
SN - 0896-6273
VL - 42
SP - 213
EP - 223
JO - Neuron
JF - Neuron
IS - 2
ER -