Conditional mutations occur predominantly in highly conserved residues of RNA polymerase II subunits

C. Scafe, C. Martin, M. Nonet, S. Podos, S. Okamura, R. A. Young

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Conditional mutations in the Saccharomyces cerevisiae RNA polymerase II large subunit, RPB1, were obtained by introducing a mutagenized RPB1 plasmid into yeast cells, selecting for loss of the wild-type RPB1 gene, and screening the cells for heat or cold sensitivity. Sequence analysis of 10 conditional RPB1 mutations and 10 conditional RPB2 mutations revealed that the amino acid residues altered by these distinct mutations are nearly always invariant among eucaryotic RPB1 and RPB2 homologs. These results suggest that RNA polymerase mutants might be obtained in other eucaryotic organisms by alteration of these invariant residues.

Original languageEnglish
Pages (from-to)1270-1275
Number of pages6
JournalMolecular and cellular biology
Volume10
Issue number3
DOIs
StatePublished - 1990

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