In the conclusion of this series of reports, the application of 31P/2H NMR to investigate the pathophysiology of sepsis in rat hindlimb muscle is demonstrated. Sepsis decreased muscle [PCr] by 18%, 18 ± 4 SD vs 22 ± 4 SD mmol/kg tissue wet wt (P = 0.01) in control rats but [ATP] was unchanged, 6 mmol/kg tissue wet wt (P = 0.2). The derived free cytosolic [ADP] in the two groups was similar, [ADP]septic = 0.023 ± 0.004 SD and [ADP]control, = 0.021 ± 0.003 SD mmol/kg tissue wet wt, and not statistically different (P =0.14). Likewise [Pi] in the septic and control groups was not statistically different, [Pi]septic = 1.1 ± 0.5 SD and [Pi]control, = 1.2 ± 0.4 SD mmol/kg tissue wet wt (P = 0.2). Septic rats presented the symptom of respiratory alkalosis evidenced by elevated blood pH. Sepsis decreased muscle blood flow by 33%, P = 0.003, but examination of individual subjects did not demonstrate a correlation with the reduction in [PCr]. Thus, a metabolic energy deficit caused by cellular ischemia/hypoxia is not a likely cause of cellular abnormality in rat hindlimb muscle during sepsis.

Original languageEnglish
Pages (from-to)67-77
Number of pages11
JournalMagnetic resonance in medicine
Issue number1
StatePublished - May 1992


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