@inbook{c1b83334ab9149f894157b894c71f5a2,
title = "Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and TLC Separation with High-Resolution Mass Spectrometry Platform",
abstract = "Lipidomic analyses by mass spectrometry (MS) of epidermal ceramides, a large family of lipids crucial to the permeability barrier of the skin, have been reported previously. To ensure the accuracy of lipid identification, we describe here the isolation of mouse newborn epidermal lipids followed by fractionation with solid-phase extraction columns, and lipidomic analyses by high-resolution MS for structural identification. We also describe here the employment of thin layer chromatography, an old but useful tool, in facilitating the structural characterization of the epidermal lipid species by MS.",
keywords = "Epidermal lipids, Mass spectrometry, Protein-bound lipids, Solid-phase extraction, Thin layer chromatography",
author = "Lin, {Meei Hua} and Miner, {Jeffrey H.} and Hsu, {Fong Fu}",
note = "Funding Information: This work was supported by NIH grant R01AR049269 to J.H.M., and NIH P30DK020579, P30DK056341, and R24GM136766 grants to Mass Spectrometry Resource of Washington University. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1410-5_10",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "139--155",
booktitle = "Methods in Molecular Biology",
}