TY - JOUR
T1 - Comprehensive identification of 125 multifarious constituents in Shuang-huang-lian powder injection by HPLC-DAD-ESI-IT-TOF-MS
AU - Sun, Hongyang
AU - Liu, Meixian
AU - Lin, Zongtao
AU - Jiang, Haixiu
AU - Niu, Yanyan
AU - Wang, Hong
AU - Chen, Shizhong
N1 - Funding Information:
This work was supported by Natural Science Foundation of Beijing, China (Grant No.7142088). And we are grateful for the proofreading of this manuscipt by Dr. Souvik Banerjee in the Department of Pharmaceutical Sciences, University of Tennessee Health Science Center.
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - A high-performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method was established for excellent separation and structural identification of constituents in Shuang-huang-lian powder injection (SHLPI). The typical ultraviolet absorptions, accurate empirical molecular formula and reasonable fragmentation mechanisms of these ingredients were used for their structural elucidation. In consequence, 125 constituents (33 phenolic acids, 29 flavonoids, 32 phenylethanoid glycosides, 15 iridoid glycosides, 8 lignans, 3 amino acids and 2 purines nucleosides, 2 quinoid glycosides and 1 alkylbenzene glycoside) were either unequivocally identified or tentatively characterized by comparing authentic standards or published data. The result showed that this study could provide valuable information for the quality control and further investigation of SHLPI formula.
AB - A high-performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) method was established for excellent separation and structural identification of constituents in Shuang-huang-lian powder injection (SHLPI). The typical ultraviolet absorptions, accurate empirical molecular formula and reasonable fragmentation mechanisms of these ingredients were used for their structural elucidation. In consequence, 125 constituents (33 phenolic acids, 29 flavonoids, 32 phenylethanoid glycosides, 15 iridoid glycosides, 8 lignans, 3 amino acids and 2 purines nucleosides, 2 quinoid glycosides and 1 alkylbenzene glycoside) were either unequivocally identified or tentatively characterized by comparing authentic standards or published data. The result showed that this study could provide valuable information for the quality control and further investigation of SHLPI formula.
KW - Flavonoids
KW - HPLC-DAD-ESI-IT-TOF-MS
KW - Iridoid glycosides
KW - Phenolic acids
KW - Phenylethanoid glycosides
KW - Shuang-huang-lian powder injection
UR - https://www.scopus.com/pages/publications/84936850788
U2 - 10.1016/j.jpba.2015.06.013
DO - 10.1016/j.jpba.2015.06.013
M3 - Article
C2 - 26177215
AN - SCOPUS:84936850788
SN - 0731-7085
VL - 115
SP - 86
EP - 106
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -