@article{6475b2526def4ef1af188b5ecaa5e348,
title = "Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH",
abstract = "Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).",
keywords = "Flow Cytometry/Mass Cytometry, Immunology, Metabolism, Microscopy, Model Organisms, Single Cell",
author = "Sabine Daemen and Chan, {Mandy M.} and Schilling, {Joel D.}",
note = "Funding Information: This work was supported by NIH grants RO1 DK11003401 (J.D.S) and ADA 118-IBS280 (J.D.S). The core services of the Diabetes Research Center and the Flow Cytometry Core at Washington University School of Medicine also supported this work. Conceptualization, S.D. M.M.C. and J.D.S.; methodology, S.D. M.M.C. and J.D.S.; investigation, S.D. M.M.C. and J.D.S.; writing-original draft, S.D. M.M.C. and J.D.S.; writing-review & editing, S.D. M.M.C. and J.D.S.; and funding Acquisition, J.D.S. The authors declare no competing interests. Funding Information: This work was supported by NIH grants RO1 DK11003401 (J.D.S) and ADA 118-IBS280 (J.D.S). The core services of the Diabetes Research Center and the Flow Cytometry Core at Washington University School of Medicine also supported this work. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100511",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
number = "2",
}