Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH

Sabine Daemen, Mandy M. Chan, Joel D. Schilling

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).

Original languageEnglish
Article number100511
JournalSTAR Protocols
Volume2
Issue number2
DOIs
StatePublished - Jun 18 2021

Keywords

  • Flow Cytometry/Mass Cytometry
  • Immunology
  • Metabolism
  • Microscopy
  • Model Organisms
  • Single Cell

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