Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH

Sabine Daemen; Mandy M. Chan; Joel D. Schilling

doi:10.1016/j.xpro.2021.100511

Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH

Sabine Daemen, Mandy M. Chan, Joel D. Schilling

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34 Scopus citations

Abstract

Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).

Original language	English
Article number	100511
Journal	STAR Protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100511
State	Published - Jun 18 2021

Keywords

Flow Cytometry/Mass Cytometry
Immunology
Metabolism
Microscopy
Model Organisms
Single Cell

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10.1016/j.xpro.2021.100511

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title = "Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH",

abstract = "Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).",

keywords = "Flow Cytometry/Mass Cytometry, Immunology, Metabolism, Microscopy, Model Organisms, Single Cell",

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AU - Daemen, Sabine

AU - Chan, Mandy M.

AU - Schilling, Joel D.

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Y1 - 2021/6/18

N2 - Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).

AB - Recently, it has become evident that macrophage diversity increases in the liver during the pathogenesis of non-alcoholic steatohepatitis (NASH). Here, we provide a detailed protocol for the analysis of liver macrophage subsets in mice with non-alcoholic fatty liver disease (NAFLD) and early NASH using flow cytometry and immunofluorescence (IF). These methods can be used to assess the composition and localization of macrophage subsets during NASH. For complete details on the use and execution of this protocol, please refer to Daemen et al. (2021).

KW - Flow Cytometry/Mass Cytometry

KW - Immunology

KW - Metabolism

KW - Microscopy

KW - Model Organisms

KW - Single Cell

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SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 2

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ER -

Comprehensive analysis of liver macrophage composition by flow cytometry and immunofluorescence in murine NASH

Abstract

Keywords

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