Complex responses activated by ibotenate in postnatal rat hippocampal neurons

Charles F. Zorumski; Richard D. Todd; David B. Clifford

doi:10.1016/0006-8993(89)90163-7

Complex responses activated by ibotenate in postnatal rat hippocampal neurons

Charles F. Zorumski, Richard D. Todd, David B. Clifford

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Abstract

The actions of the excitatory amino acid ibotenate were investigated in postnatal rat hippocampal neurons. In neurons voltage clamped at negative membrane potentials using low-chloride internal solutions, ibotenate responses consist of an inward cationic current and two outward currents. The inward current is inhibited by 2-amino-5-phosphonovalerate (APV), a selective N-methyl-d-aspartate (NMDA) antagonist. The two outward currents consist of a picrotoxin and bicuculline-sensitive chloride current and a slowly developing calcium activated potassium current. The bicuculline sensitive current appears to be the product of contamination of ibotenate samples with the γ-aminobutyric acid (GABA) agonist muscimol and not the result of a direct action of ibotenate.

Original language	English
Pages (from-to)	193-197
Number of pages	5
Journal	Brain Research
Volume	494
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(89)90163-7
State	Published - Aug 7 1989

Keywords

Chloride current
Ibotenate
Muscimol
N-Methyl-d-aspartate
Voltage clamp

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10.1016/0006-8993(89)90163-7

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title = "Complex responses activated by ibotenate in postnatal rat hippocampal neurons",

abstract = "The actions of the excitatory amino acid ibotenate were investigated in postnatal rat hippocampal neurons. In neurons voltage clamped at negative membrane potentials using low-chloride internal solutions, ibotenate responses consist of an inward cationic current and two outward currents. The inward current is inhibited by 2-amino-5-phosphonovalerate (APV), a selective N-methyl-d-aspartate (NMDA) antagonist. The two outward currents consist of a picrotoxin and bicuculline-sensitive chloride current and a slowly developing calcium activated potassium current. The bicuculline sensitive current appears to be the product of contamination of ibotenate samples with the γ-aminobutyric acid (GABA) agonist muscimol and not the result of a direct action of ibotenate.",

keywords = "Chloride current, Ibotenate, Muscimol, N-Methyl-d-aspartate, Voltage clamp",

author = "Zorumski, {Charles F.} and Todd, {Richard D.} and Clifford, {David B.}",

note = "Funding Information: This work was supported in part by NIMH Physician Scientist Award MH00630, the Klingen-stein Foundation and the Bantly Foundation (C.EZ.). The authors thank Liu Lin Thio and Gary D. Clark for many helpful discussions.",

year = "1989",

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doi = "10.1016/0006-8993(89)90163-7",

language = "English",

volume = "494",

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journal = "Brain Research",

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T1 - Complex responses activated by ibotenate in postnatal rat hippocampal neurons

AU - Zorumski, Charles F.

AU - Todd, Richard D.

AU - Clifford, David B.

N1 - Funding Information: This work was supported in part by NIMH Physician Scientist Award MH00630, the Klingen-stein Foundation and the Bantly Foundation (C.EZ.). The authors thank Liu Lin Thio and Gary D. Clark for many helpful discussions.

PY - 1989/8/7

Y1 - 1989/8/7

N2 - The actions of the excitatory amino acid ibotenate were investigated in postnatal rat hippocampal neurons. In neurons voltage clamped at negative membrane potentials using low-chloride internal solutions, ibotenate responses consist of an inward cationic current and two outward currents. The inward current is inhibited by 2-amino-5-phosphonovalerate (APV), a selective N-methyl-d-aspartate (NMDA) antagonist. The two outward currents consist of a picrotoxin and bicuculline-sensitive chloride current and a slowly developing calcium activated potassium current. The bicuculline sensitive current appears to be the product of contamination of ibotenate samples with the γ-aminobutyric acid (GABA) agonist muscimol and not the result of a direct action of ibotenate.

AB - The actions of the excitatory amino acid ibotenate were investigated in postnatal rat hippocampal neurons. In neurons voltage clamped at negative membrane potentials using low-chloride internal solutions, ibotenate responses consist of an inward cationic current and two outward currents. The inward current is inhibited by 2-amino-5-phosphonovalerate (APV), a selective N-methyl-d-aspartate (NMDA) antagonist. The two outward currents consist of a picrotoxin and bicuculline-sensitive chloride current and a slowly developing calcium activated potassium current. The bicuculline sensitive current appears to be the product of contamination of ibotenate samples with the γ-aminobutyric acid (GABA) agonist muscimol and not the result of a direct action of ibotenate.

KW - Chloride current

KW - Ibotenate

KW - Muscimol

KW - N-Methyl-d-aspartate

KW - Voltage clamp

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U2 - 10.1016/0006-8993(89)90163-7

DO - 10.1016/0006-8993(89)90163-7

M3 - Article

C2 - 2569917

AN - SCOPUS:0024315922

SN - 0006-8993

VL - 494

SP - 193

EP - 197

JO - Brain Research

JF - Brain Research

IS - 1

ER -

Complex responses activated by ibotenate in postnatal rat hippocampal neurons

Abstract

Keywords

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