TY - JOUR
T1 - Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea
AU - Finkbeiner, Stacy R.
AU - Kirkwood, Carl D.
AU - Wang, David
N1 - Funding Information:
This work was funded in part by an NHMRC RD Wright Research Fellowship (ID 334364, CK), and by the Food Safety Research Response Network, a Coordinated Agricultural Project, funded through the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number ##2005-35212-15287.
PY - 2008
Y1 - 2008
N2 - Background. Astroviruses infect a variety of mammals and birds and are causative agents of diarrhea in humans and other animal hosts. We have previously described the identification of several sequence fragments with limited sequence identity to known astroviruses in a stool specimen obtained from a child with acute diarrhea, suggesting that a novel virus was present. Results. In this study, the complete genome of this novel virus isolate was sequenced and analyzed. The overall genome organization of this virus paralleled that of known astroviruses, with 3 open reading frames identified. Phylogenetic analysis of the ORFs indicated that this virus is highly divergent from all previously described animal and human astroviruses. Molecular features that are highly conserved in human serotypes 1-8, such as a 3′NTR stem-loop structure and conserved nucleotide motifs present in the 5′NTR and ORF1b/2 junction, were either absent or only partially conserved in this novel virus. Conclusion. Based on the analyses described herein, we propose that this newly discovered virus represents a novel species in the family Astroviridae. It has tentatively been named Astrovirus MLB1.
AB - Background. Astroviruses infect a variety of mammals and birds and are causative agents of diarrhea in humans and other animal hosts. We have previously described the identification of several sequence fragments with limited sequence identity to known astroviruses in a stool specimen obtained from a child with acute diarrhea, suggesting that a novel virus was present. Results. In this study, the complete genome of this novel virus isolate was sequenced and analyzed. The overall genome organization of this virus paralleled that of known astroviruses, with 3 open reading frames identified. Phylogenetic analysis of the ORFs indicated that this virus is highly divergent from all previously described animal and human astroviruses. Molecular features that are highly conserved in human serotypes 1-8, such as a 3′NTR stem-loop structure and conserved nucleotide motifs present in the 5′NTR and ORF1b/2 junction, were either absent or only partially conserved in this novel virus. Conclusion. Based on the analyses described herein, we propose that this newly discovered virus represents a novel species in the family Astroviridae. It has tentatively been named Astrovirus MLB1.
UR - http://www.scopus.com/inward/record.url?scp=55149116731&partnerID=8YFLogxK
U2 - 10.1186/1743-422X-5-117
DO - 10.1186/1743-422X-5-117
M3 - Article
C2 - 18854035
AN - SCOPUS:55149116731
SN - 1743-422X
VL - 5
JO - Virology Journal
JF - Virology Journal
M1 - 117
ER -