Complement Protein C1q Inhibits Antibody-Dependent Enhancement of Flavivirus Infection in an IgG Subclass-Specific Manner

Erin Mehlhop, Camilo Ansarah-Sobrinho, Syd Johnson, Michael Engle, Daved H. Fremont, Theodore C C. Pierson, Michael S. Diamond

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Severe dengue virus infection can occur in humans with pre-existing antibodies against the virus. This observation led to the hypothesis that a subneutralizing antibody level in vivo can increase viral burden and cause more severe disease. Indeed, antibody-dependent enhancement of infection (ADE) in vitro has been described for multiple viruses, including the flaviviruses dengue virus and West Nile virus. Here, we demonstrate that the complement component C1q restricts ADE by anti-flavivirus IgG antibodies in an IgG subclass-specific manner in cell culture and in mice. IgG subclasses that avidly bind C1q induced minimal ADE in the presence of C1q. These findings add a layer of complexity for the analysis of humoral immunity and flavivirus infection.

Original languageEnglish
Pages (from-to)417-426
Number of pages10
JournalCell Host and Microbe
Volume2
Issue number6
DOIs
StatePublished - Dec 13 2007

Keywords

  • MICROBIO
  • MOLIMMUNO

Fingerprint Dive into the research topics of 'Complement Protein C1q Inhibits Antibody-Dependent Enhancement of Flavivirus Infection in an IgG Subclass-Specific Manner'. Together they form a unique fingerprint.

  • Cite this