TY - JOUR
T1 - Complement biosynthesis by human bronchoalveolar macrophages
AU - Cole, F. Sessions
AU - Matthews, Wallace J.
AU - Rossing, Thomas H.
AU - Gash, David J.
AU - Lichtenberg, Nancy A.
AU - Pennington, James E.
N1 - Funding Information:
’ This work was supported in part by United States Public Health Service Grants HL 21997, HL 22487, and AI 15033: the Ina Sue Perlmutter Cystic Fibrosis Research Fund: a National Research Service Award AI 06178-01 (FSC): a Research Starter Grant (FSC) from the Charles H. Hood Foundation; and a Parker B. Francis Fellowship (THR).
PY - 1983/5
Y1 - 1983/5
N2 - Complement production by bronchoalveolar macrophages recovered from 8 normal volunteers and 15 patients with a variety of lung diseases was measured functionally and immunochemically. While macrophages from all eight normals demonstrated the capacity to secrete hemolytically active C2 and factor B within 48 hr of culture at consistent rates, bronchoalveolar macrophages from patients secreted C2 and factor B in widely differing amounts, and in some cases, not at all. No functional, secreted C3 was detected from normal macrophage monolayers, although apparently native C3 protein was synthesized and secreted. In contrast, functional C3 was produced by macrophage monolayers from 3 of 15 patients. These findings suggest that complement production by the normal human bronchoalveolar macrophage differs from its progenitor cell, the blood monocyte, and that complement production by bronchoalveolar macrophages may be altered in different pulmonary diseases.
AB - Complement production by bronchoalveolar macrophages recovered from 8 normal volunteers and 15 patients with a variety of lung diseases was measured functionally and immunochemically. While macrophages from all eight normals demonstrated the capacity to secrete hemolytically active C2 and factor B within 48 hr of culture at consistent rates, bronchoalveolar macrophages from patients secreted C2 and factor B in widely differing amounts, and in some cases, not at all. No functional, secreted C3 was detected from normal macrophage monolayers, although apparently native C3 protein was synthesized and secreted. In contrast, functional C3 was produced by macrophage monolayers from 3 of 15 patients. These findings suggest that complement production by the normal human bronchoalveolar macrophage differs from its progenitor cell, the blood monocyte, and that complement production by bronchoalveolar macrophages may be altered in different pulmonary diseases.
UR - http://www.scopus.com/inward/record.url?scp=0020595396&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(83)90065-X
DO - 10.1016/0090-1229(83)90065-X
M3 - Article
C2 - 6553519
AN - SCOPUS:0020595396
SN - 0090-1229
VL - 27
SP - 153
EP - 159
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 2
ER -