Comparison of rat pineal gland and rat liver serotonin-n-acetyltransferase

Jeremiah J. Morrissey, Scott B. Edwards, Walter Lovenberg

Research output: Contribution to journalArticlepeer-review


The kinetic and molecular weight characteristics of the non-inducible rat liver and the inducible rat pineal gland N-acetyltransferases were determined and compared. Both enzymes exhibited initial rate kinetics suggestive of a ping-pong mechanism implying the existence of an acetylated enzyme intermediate. The elution characteristics of the enzyme activities on Sephadex G-100 indicate an apparent molecular weight for the liver enzyme of 26,000 while the pineal gland N-acetyltransferase eluted at an apparent weight of 39,000. Molecular weight determinations performed in the presence of β-mercaptoethylamine suggest active subunit molecular weights of 10,000 - 12,000 daltons for the pineal gland and liver enzymes, respectively.

Original languageEnglish
Pages (from-to)118-123
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Jul 11 1977


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