TY - JOUR
T1 - Comparison of rat pineal gland and rat liver serotonin-n-acetyltransferase
AU - Morrissey, Jeremiah J.
AU - Edwards, Scott B.
AU - Lovenberg, Walter
PY - 1977/7/11
Y1 - 1977/7/11
N2 - The kinetic and molecular weight characteristics of the non-inducible rat liver and the inducible rat pineal gland N-acetyltransferases were determined and compared. Both enzymes exhibited initial rate kinetics suggestive of a ping-pong mechanism implying the existence of an acetylated enzyme intermediate. The elution characteristics of the enzyme activities on Sephadex G-100 indicate an apparent molecular weight for the liver enzyme of 26,000 while the pineal gland N-acetyltransferase eluted at an apparent weight of 39,000. Molecular weight determinations performed in the presence of β-mercaptoethylamine suggest active subunit molecular weights of 10,000 - 12,000 daltons for the pineal gland and liver enzymes, respectively.
AB - The kinetic and molecular weight characteristics of the non-inducible rat liver and the inducible rat pineal gland N-acetyltransferases were determined and compared. Both enzymes exhibited initial rate kinetics suggestive of a ping-pong mechanism implying the existence of an acetylated enzyme intermediate. The elution characteristics of the enzyme activities on Sephadex G-100 indicate an apparent molecular weight for the liver enzyme of 26,000 while the pineal gland N-acetyltransferase eluted at an apparent weight of 39,000. Molecular weight determinations performed in the presence of β-mercaptoethylamine suggest active subunit molecular weights of 10,000 - 12,000 daltons for the pineal gland and liver enzymes, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0017377986&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(77)80172-1
DO - 10.1016/S0006-291X(77)80172-1
M3 - Article
C2 - 883970
AN - SCOPUS:0017377986
SN - 0006-291X
VL - 77
SP - 118
EP - 123
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -