Comparison of RAG gene expression in normal and transformed precursor lymphocytes

Gary Rathbun, Eugene M. Oitz, Frederick W. Alt

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Analyses of mechanisms that regulate V(D)J recombination have relied heavily on the use of transformed precursor lymphocyte cell lines. We now show that such lines have highly variable and frequently low levels of recombination activating genes (RAG)-1 and -2 gene expression. We also show that expression levels of the RAG genes can vary > 100-fold between different subcloned cells of an individual pre-B line. We discuss these findings in the context of normal regulation of RAG gene expression and the implication for the use of transformed pre-B cell lines as models for studying control of V(D)J recombination activity.

Original languageEnglish
Pages (from-to)997-1000
Number of pages4
JournalInternational Immunology
Volume5
Issue number8
DOIs
StatePublished - Aug 1993

Keywords

  • Cell-based model systems
  • Lymphoid progenitors
  • V(D)J recombinase activity

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