Comparison of hyperpolarized 13C and non-hyperpolarized deuterium MRI approaches for imaging cerebral glucose metabolism at 4.7 T

Cornelius von Morze, John A. Engelbach, Tyler Blazey, James D. Quirk, Galen D. Reed, Joseph E. Ippolito, Joel R. Garbow

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Purpose: The purpose of this study was to directly compare two isotopic metabolic imaging approaches, hyperpolarized (HP) 13C MRI and deuterium metabolic imaging (DMI), for imaging specific closely related segments of cerebral glucose metabolism at 4.7 T. Methods: Comparative HP-13C and DMI neuroimaging experiments were conducted consecutively in normal rats during the same scanning session. Localized conversions of [1-13C]pyruvate and [6,6-2H2]glucose to their respective downstream metabolic products were measured by spectroscopic imaging, using an identical 2D-CSI sequence with parameters optimized for the respective experiments. To facilitate direct comparison, a pair of substantially equivalent 2.5-cm double-tuned X/1H RF surface coils was developed. For improved results, multidimensional low-rank reconstruction was applied to denoise the raw DMI data. Results: Localized conversion of HP [1-13C]pyruvate to [1-13C]lactate, and [6,6-2H2]glucose to [3,3-2H2]lactate and Glx-d (glutamate and glutamine), was detected in rat brain by spectroscopic imaging at 4.7 T. The SNR and spatial resolution of HP-13C MRI was superior to DMI but limited to a short time window, whereas the lengthy DMI acquisition yielded maps of not only lactate, but also Glx production, albeit with relatively poor spectral discrimination between metabolites at this field strength. Across the individual rats, there was an apparent inverse correlation between cerebral production of HP [1-13C]lactate and Glx-d, along with a trend toward increased [3,3-2H2]lactate. Conclusion: The HP-13C MRI and DMI methods are both feasible at 4.7 T and have significant potential for metabolic imaging of specific segments of glucose metabolism.

Original languageEnglish
Pages (from-to)1795-1804
Number of pages10
JournalMagnetic resonance in medicine
Volume85
Issue number4
DOIs
StatePublished - Apr 2021

Keywords

  • brain
  • dynamic nuclear polarization
  • imaging
  • stable isotopes

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