TY - JOUR
T1 - Comparison of humoral immune responses to epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus using a viral proteome microarray
AU - Zheng, Dasheng
AU - Wan, Jun
AU - Cho, Yong Gu
AU - Wang, Leyao
AU - Chiou, Chuang Jiun
AU - Pai, Shweta
AU - Woodard, Crystal
AU - Zhu, Jian
AU - Liao, Gangling
AU - Martinez-Maza, Otoniel
AU - Qian, Jiang
AU - Zhu, Heng
AU - Hayward, Gary S.
AU - Ambinder, Richard F.
AU - Hayward, S. Diane
N1 - Funding Information:
Financial support. This work was supported by Public Health Service grants from the National Cancer Institute of the National Institutes of Health (R37 CA42245 and R01 CA30356 to S. D. H, R21 CA138163 to S. D. H. and H. Z., RC2 CA148402 to G. S. H., and Cancer Center Core Grant P30CA006973 to William Nelson). Potential conflicts of interest. All authors: No reported conflicts.
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.
AB - Background: Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and Kaposi's sarcoma-associated herpesvirus (KSHV) has a restricted seroprevalence. Both viruses are associated with malignancies that have an increased frequency in individuals who are coinfected with human immunodeficiency virus type 1 (HIV-1).Methods.To obtain an overview of humoral immune responses to these viruses, we generated a protein array that displayed 174 EBV and KSHV polypeptides purified from yeast. Antibody responses to EBV and KSHV were examined in plasma from healthy volunteers and patients with B cell lymphoma or with AIDS-related Kaposi's sarcoma or lymphoma.Results.In addition to the commonly studied antigens, IgG responses were frequently detected to the tegument proteins KSHV ORF38 and EBV BBRF and BGLF2 and BNRF1 and to the EBV early lytic proteins BRRF1 and BORF2. The EBV vIL-10 protein was particularly well recognized by plasma IgA. The most intense IgG responses to EBV antigens occurred in HIV-1-positive patients. No clear correlation was observed between viral DNA load in plasma and antibody profile.Conclusions.The protein array provided a sensitive platform for global screening; identified new, frequently recognized viral antigens; and revealed a broader humoral response to EBV compared with KSHV in the same patients.
UR - http://www.scopus.com/inward/record.url?scp=80155198393&partnerID=8YFLogxK
U2 - 10.1093/infdis/jir645
DO - 10.1093/infdis/jir645
M3 - Article
C2 - 21990424
AN - SCOPUS:80155198393
SN - 0022-1899
VL - 204
SP - 1683
EP - 1691
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 11
ER -