Comparison of gene expression profiles between human and mouse monocyte subsets

Molly A. Ingersoll, Rainer Spanbroek, Claudio Lottaz, Emmanuel L. Gautier, Marion Frankenberger, Reinhard Hoffmann, Roland Lang, Muzlifah Haniffa, Matthew Collin, Frank Tacke, Andreas J.R. Habenicht, Loems Ziegler-Heitbrock, Gwendalyn J. Randolph

Research output: Contribution to journalArticlepeer-review

541 Scopus citations


Blood of both humans and mice contains 2 main monocyte subsets. Here, we investigated the extent of their similarity using a microarray approach. Approximately 270 genes in humans and 550 genes in mice were differentially expressed between subsets by 2-fold or more. More than 130 of these gene expression differences were conserved between mouse and human monocyte subsets. We confirmed numerous of these differences at the cell surface protein level. Despite overall conservation, some molecules were conversely expressed between the 2 species' subsets, including CD36, CD9, and TREM-1. Other differences included a prominent peroxisome proliferatoractivated receptor γ (PPARγ) signature in mouse monocytes, which is absent in humans, and strikingly opposed patterns of receptors involved in uptake of apoptotic cells and other phagocytic cargo between human and mouse monocyte subsets. Thus, whereas human and mouse monocyte subsets are far more broadly conserved than currently recognized, important differences between the species deserve consideration when models of human disease are studied in mice.

Original languageEnglish
Pages (from-to)e10-e19
Issue number3
StatePublished - Jan 21 2010


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