TY - JOUR
T1 - Comparison of five antibodies as markers in the diagnosis of melanoma in cytologic preparations
AU - Sheffield, Matthew V.
AU - Yee, Herman
AU - Dorvault, Christine C.
AU - Weilbaecher, Katherine N.
AU - Eltoum, Isam A.
AU - Siegal, Gene P.
AU - Fisher, David E.
AU - Chhieng, David C.
PY - 2002/12/1
Y1 - 2002/12/1
N2 - We determined the sensitivity and specificity of 3 novel antibodies (microphthalmia transcription factor [Mitf], Melan-A, and tyrosinase) as markers for melanoma in cytologic preparations and compared the results with those of commonly used markers (S-100 protein [S-100] and HMB-45). We stained 72 cell blocks from 40 patients with melanoma and 32 with nonmelanocytic malignant neoplasms with antibodies against S-100, HMB-45, Mitf, Melan-A, and tyrosinase. Histologic correlation was available in more than 95% of cases. Nuclear staining for Mitf and cytoplasmic staining for S-100, HMB-45, Melan-A, and tyrosinase in more than 10% of tumor cells was considered positive. All 3 novel markers demonstrated sensitivity superior to S-100 and HMB-45. HMB-45, Melan-A, and Mitf demonstrated specificities of 97%. S-100 protein and tyrosinase were less specific. Sensitivity and specificity for the combination Mitf+/Melan-A+ were 95% and 100%, respectively, whereas they were 80% and 100%, respectively, for S-100+/HMB-45+. Mitf, Melan-A, and tyrosinase are sensitive markers for epithelioid melanoma. Milf and Melan-A seem more specific than S-100 and tyrosinase. An antibody panel consisting of Mitf and Melan-A is superior to a panel of S-100 and HMB-45 in the diagnosis of melanoma in cytologic specimens.
AB - We determined the sensitivity and specificity of 3 novel antibodies (microphthalmia transcription factor [Mitf], Melan-A, and tyrosinase) as markers for melanoma in cytologic preparations and compared the results with those of commonly used markers (S-100 protein [S-100] and HMB-45). We stained 72 cell blocks from 40 patients with melanoma and 32 with nonmelanocytic malignant neoplasms with antibodies against S-100, HMB-45, Mitf, Melan-A, and tyrosinase. Histologic correlation was available in more than 95% of cases. Nuclear staining for Mitf and cytoplasmic staining for S-100, HMB-45, Melan-A, and tyrosinase in more than 10% of tumor cells was considered positive. All 3 novel markers demonstrated sensitivity superior to S-100 and HMB-45. HMB-45, Melan-A, and Mitf demonstrated specificities of 97%. S-100 protein and tyrosinase were less specific. Sensitivity and specificity for the combination Mitf+/Melan-A+ were 95% and 100%, respectively, whereas they were 80% and 100%, respectively, for S-100+/HMB-45+. Mitf, Melan-A, and tyrosinase are sensitive markers for epithelioid melanoma. Milf and Melan-A seem more specific than S-100 and tyrosinase. An antibody panel consisting of Mitf and Melan-A is superior to a panel of S-100 and HMB-45 in the diagnosis of melanoma in cytologic specimens.
KW - Cytology
KW - HMB-45
KW - Immunocytochemistry
KW - Melan-A
KW - Melanoma
KW - Microphthalmia transcription factor
KW - S-100
KW - Tyrosinase
UR - http://www.scopus.com/inward/record.url?scp=0036884929&partnerID=8YFLogxK
U2 - 10.1309/EWK9-LUPR-6BC5-1GXV
DO - 10.1309/EWK9-LUPR-6BC5-1GXV
M3 - Article
C2 - 12472287
AN - SCOPUS:0036884929
SN - 0002-9173
VL - 118
SP - 930
EP - 936
JO - American journal of clinical pathology
JF - American journal of clinical pathology
IS - 6
ER -