Comparison of endolymph cross-sectional area measured histologically with that measured in vivo with an ionic volume marker

Alec N. Salt, John E. DeMott, Robert S. Kimura

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

In order to establish how endolymph volume is regulated, it is essential to be able to measure endolymph volume or cross-sectional area in vivo. We have developed methods to accomplish this by injecting the volume marker ion hexafluoroarsenate (AsF6) into endolymph by iontophoresis. For an injection at a constant rate, the endolymph concentration is inversely dependent on the cross-sectional area of the scala into which injection occurred. Marker concentrations were monitored by inserting ion-selective microelectrodes into endolymph near the injection site. In a previous study we quantified the degree of hydrops in animals following ablation of the endolymphatic sac. In the present study we validated the technique by comparing the endolymphatic cross-sectional area measured in vivo with AsF6 with that measured by established histologic procedures. The correlation between the two measures was good, with a coefficient of .903, although the area measured histologically was a little lower than that measured in vivo.

Original languageEnglish
Pages (from-to)886-894
Number of pages9
JournalAnnals of Otology, Rhinology & Laryngology
Volume104
Issue number11
DOIs
StatePublished - Nov 1995

Keywords

  • Meniere's disease
  • endolymph
  • endolymphatic hydrops
  • hexafluoroarsenate

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