Comparative study of the complement activating and specific IgE-binding properties of ragweed pollen allergen

T. Hidvégi, L. Berrens, L. Varga, F. Marañon, B. Schmidt, M. Kirschfink, G. Füst

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Previous reports have defined the capacity of ragweed pollen extract (RWA) to activate human complement (C) in fluid phase through the classical pathway and have ascertained a strong correlation between the extent of complement activation and the severity of symptoms of allergic rhinoconjunctivitis during the ragweed blooming season. In the present study the complement-activating and specific IgE-binding capacities of various ragweed allergen preparations were compared. Elimination of physically adsorbed (flavonoid) pigments from the allergenic proteins had no significant effect on their complement-consuming capacity, although the process strongly diminished specific IgE binding. Removal of an IgE-binding trypsin inhibitor from RWA significantly enhanced RWA-induced complement activation, whereas it did not change IgE binding. These findings indicate that neither the physically adsorbed pigments nor the trypsin inhibitor are involved in complement activation by ragweed pollen allergens, and suggest that complement activation and specific IgE binding are distinct molecular properties of ragweed pollen allergen.

Original languageEnglish
Pages (from-to)122-127
Number of pages6
JournalClinical and Experimental Immunology
Issue number1
StatePublished - 1997


  • Allergens
  • Complement
  • Flavonoids
  • Ragweed pollen
  • Trypsin inhibitor


Dive into the research topics of 'Comparative study of the complement activating and specific IgE-binding properties of ragweed pollen allergen'. Together they form a unique fingerprint.

Cite this