TY - JOUR
T1 - Comparative analysis of USA300 virulence determinants in a rabbit model of skin and soft tissue infection
AU - Kobayashi, Scott D.
AU - Malachowa, Natalia
AU - Whitney, Adeline R.
AU - Braughton, Kevin R.
AU - Gardner, Donald J.
AU - Long, Dan
AU - Wardenburg, Juliane Bubeck
AU - Schneewind, Olaf
AU - Otto, Michael
AU - DeLeo, Frank R.
N1 - Funding Information:
This work was supported in part by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH). J. B. W. and O. S. acknowledge membership in and support from the Region V Great Lakes Regional Center of Excellence (RCE) (NIH award 2-U54-AI-057153).
PY - 2011/9/15
Y1 - 2011/9/15
N2 - Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton- Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-solublemodulin-alpha peptides (PSMα), and accessory gene regulator (Agr). The data indicate that Hla, PSMα, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected.
AB - Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are frequently associated with strains harboring genes encoding Panton- Valentine leukocidin (PVL). The role of PVL in the success of the epidemic CAMRSA strain USA300 remains unknown. Here we developed a skin and soft tissue infection model in rabbits to test the hypothesis that PVL contributes to USA300 pathogenesis and compare it with well-established virulence determinants: alpha-hemolysin (Hla), phenol-solublemodulin-alpha peptides (PSMα), and accessory gene regulator (Agr). The data indicate that Hla, PSMα, and Agr contribute to the pathogenesis of USA300 skin infections in rabbits, whereas a role for PVL could not be detected.
UR - https://www.scopus.com/pages/publications/80052221257
U2 - 10.1093/infdis/jir441
DO - 10.1093/infdis/jir441
M3 - Article
C2 - 21849291
AN - SCOPUS:80052221257
SN - 0022-1899
VL - 204
SP - 937
EP - 941
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -