Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics

Haojia Wu; Kohei Uchimura; Erinn L. Donnelly; Yuhei Kirita; Samantha A. Morris; Benjamin D. Humphreys

doi:10.1016/j.stem.2018.10.010

Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics

Haojia Wu
, Kohei Uchimura
, Erinn L. Donnelly
, Yuhei Kirita
, Samantha A. Morris
, Benjamin D. Humphreys

Research output: Contribution to journal › Article › peer-review

479 Scopus citations

Abstract

A strong characterization of cell types, lineages, and differentiation states present in human PSC-derived kidney organoids is critical to improve differentiation protocols. Wu et al. use single-cell transcriptomics to reveal non-renal cell types, describe lineage-specific expression of regulatory genes, and report a broadly applicable strategy to reduce off-target cell populations.

Original language	English
Pages (from-to)	869-881.e8
Journal	Cell Stem Cell
Volume	23
Issue number	6
DOIs	https://doi.org/10.1016/j.stem.2018.10.010
State	Published - Dec 6 2018

Keywords

BDNF signaling
Dropseq
human kidney
induced pluripotent stem cells
kidney organoid
off-target cells
single cell RNA-seq
stem cell differentiation

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10.1016/j.stem.2018.10.010

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title = "Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics",

abstract = "A strong characterization of cell types, lineages, and differentiation states present in human PSC-derived kidney organoids is critical to improve differentiation protocols. Wu et al. use single-cell transcriptomics to reveal non-renal cell types, describe lineage-specific expression of regulatory genes, and report a broadly applicable strategy to reduce off-target cell populations.",

keywords = "BDNF signaling, Dropseq, human kidney, induced pluripotent stem cells, kidney organoid, off-target cells, single cell RNA-seq, stem cell differentiation",

author = "Haojia Wu and Kohei Uchimura and Donnelly, \{Erinn L.\} and Yuhei Kirita and Morris, \{Samantha A.\} and Humphreys, \{Benjamin D.\}",

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AU - Donnelly, Erinn L.

AU - Kirita, Yuhei

AU - Morris, Samantha A.

AU - Humphreys, Benjamin D.

PY - 2018/12/6

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KW - BDNF signaling

KW - Dropseq

KW - human kidney

KW - induced pluripotent stem cells

KW - kidney organoid

KW - off-target cells

KW - single cell RNA-seq

KW - stem cell differentiation

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ER -

Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics

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Keywords

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