Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics

Haojia Wu, Kohei Uchimura, Erinn L. Donnelly, Yuhei Kirita, Samantha A. Morris, Benjamin D. Humphreys

Research output: Contribution to journalArticlepeer-review

262 Scopus citations

Abstract

A strong characterization of cell types, lineages, and differentiation states present in human PSC-derived kidney organoids is critical to improve differentiation protocols. Wu et al. use single-cell transcriptomics to reveal non-renal cell types, describe lineage-specific expression of regulatory genes, and report a broadly applicable strategy to reduce off-target cell populations.

Original languageEnglish
Pages (from-to)869-881.e8
JournalCell Stem Cell
Volume23
Issue number6
DOIs
StatePublished - Dec 6 2018

Keywords

  • BDNF signaling
  • Dropseq
  • human kidney
  • induced pluripotent stem cells
  • kidney organoid
  • off-target cells
  • single cell RNA-seq
  • stem cell differentiation

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