Combining genomic and proteomic approaches for epigenetics research

Yumiao Han, Benjamin A. Garcia

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations

Abstract

Epigenetics is the study of changes in gene expression or cellular phenotype that do not change the DNA sequence. In this review, current methods, both genomic and proteomic, associated with epigenetics research are discussed. Among them, chromatin immunoprecipitation (ChIP) followed by sequencing and other ChIP-based techniques are powerful techniques for genome-wide profiling of DNA-binding proteins, histone post-translational modifications or nucleosome positions. However, mass spectrometry-based proteomics is increasingly being used in functional biological studies and has proved to be an indispensable tool to characterize histone modifications, as well as DNA-protein and protein-protein interactions. With the development of genomic and proteomic approaches, combination of ChIP and mass spectrometry has the potential to expand our knowledge of epigenetics research to a higher level.

Original languageEnglish
Pages (from-to)439-452
Number of pages14
JournalEpigenomics
Volume5
Issue number4
DOIs
StatePublished - Aug 2013

Keywords

  • approach
  • chromatin immunoprecipitation
  • DNA methylation
  • epigenetic
  • genomic
  • histone modification
  • mass spectrometry
  • proteomic

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