Combined optical and mechanical scanning in optical-resolution photoacoustic microscopy

  • Lei Li
  • , Chenghung Yeh
  • , Song Hu
  • , Lidai Wang
  • , Brian T. Soetikno
  • , Ruimin Chen
  • , Qifa Zhou
  • , K. Kirk Shung
  • , Konstantin I. Maslov
  • , Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Combined optical and mechanical scanning (COMS) in optical-resolution photoacoustic microscopy (OR-PAM) has provided five scanning modes with fast imaging speed and wide field of view (FOV). With two-dimensional (2D) galvanometer-based optical scanning, we have achieved a 2 KHz B-scan rate and 50 Hz volumetric-scan rate, which enables real-time tracking of cell activities in vivo. With optical-mechanical hybrid 2D scanning, we are able to image a wide FOV (10×8 mm2) within 150 seconds, which is 20 times faster than the conventional mechanical scan in our second-generation OR-PAM. With three-dimensional mechanical-based contour scanning, we can maintain the optimal signal-to-noise ratio and spatial resolution of OR-PAM while imaging objects with uneven surfaces, which is ideal for fast and quantitative studies of tumors and the brain.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2014
PublisherSPIE
ISBN (Print)9780819498564
DOIs
StatePublished - 2014
EventPhotons Plus Ultrasound: Imaging and Sensing 2014 - San Francisco, CA, United States
Duration: Feb 2 2014Feb 5 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8943
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2014
Country/TerritoryUnited States
CitySan Francisco, CA
Period02/2/1402/5/14

Keywords

  • Medical and biological imaging
  • Photoacoustic imaging
  • Scanning microscopy
  • Three-dimensional microscopy

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