Combined liquid chromatography-mass spectrometry for microscale structural studies of carbohydrates

Fong Fu Hsu; Charles G. Edmonds; James A. McCloskey

doi:10.1080/00032718608066299

Combined liquid chromatography-mass spectrometry for microscale structural studies of carbohydrates

Fong Fu Hsu, Charles G. Edmonds, James A. McCloskey

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13 Scopus citations

Abstract

Combined HPLC-thermospray mass spectrometry of mono-and disaccharides, 1-0-methylglycosides and O-permethyl mono-through tetrasaccharides has been studied to assess the potential role ol thermospray ionization for microscale structural studies of saccharides and glycoconjugates, and for high sensitivity detection of liquid chromatographic effluents. Using NH₄CO₂H as eluant for reversed-phase HPLC, abundant MNH₄⁺ions are formed from monosaccharides and mono-and per-methylated saccharides, and are suitable for monitoring sub-nanogram constituents in HPLC effluents. Detection of 100 pg (0.5 pmole) of 1-0-methylhexopyranosides with signal/noise >10 is demonstrated.

Original language	English
Pages (from-to)	1259-1271
Number of pages	13
Journal	Analytical Letters
Volume	19
Issue number	11-12
DOIs	https://doi.org/10.1080/00032718608066299
State	Published - Jan 1986

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title = "Combined liquid chromatography-mass spectrometry for microscale structural studies of carbohydrates",

abstract = "Combined HPLC-thermospray mass spectrometry of mono-and disaccharides, 1-0-methylglycosides and O-permethyl mono-through tetrasaccharides has been studied to assess the potential role ol thermospray ionization for microscale structural studies of saccharides and glycoconjugates, and for high sensitivity detection of liquid chromatographic effluents. Using NH4CO2H as eluant for reversed-phase HPLC, abundant MNH4+ions are formed from monosaccharides and mono-and per-methylated saccharides, and are suitable for monitoring sub-nanogram constituents in HPLC effluents. Detection of 100 pg (0.5 pmole) of 1-0-methylhexopyranosides with signal/noise >10 is demonstrated.",

author = "Hsu, {Fong Fu} and Edmonds, {Charles G.} and McCloskey, {James A.}",

note = "Funding Information: This work was supported by the National Institute o f General",

year = "1986",

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doi = "10.1080/00032718608066299",

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T1 - Combined liquid chromatography-mass spectrometry for microscale structural studies of carbohydrates

AU - Hsu, Fong Fu

AU - Edmonds, Charles G.

AU - McCloskey, James A.

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PY - 1986/1

Y1 - 1986/1

N2 - Combined HPLC-thermospray mass spectrometry of mono-and disaccharides, 1-0-methylglycosides and O-permethyl mono-through tetrasaccharides has been studied to assess the potential role ol thermospray ionization for microscale structural studies of saccharides and glycoconjugates, and for high sensitivity detection of liquid chromatographic effluents. Using NH4CO2H as eluant for reversed-phase HPLC, abundant MNH4+ions are formed from monosaccharides and mono-and per-methylated saccharides, and are suitable for monitoring sub-nanogram constituents in HPLC effluents. Detection of 100 pg (0.5 pmole) of 1-0-methylhexopyranosides with signal/noise >10 is demonstrated.

AB - Combined HPLC-thermospray mass spectrometry of mono-and disaccharides, 1-0-methylglycosides and O-permethyl mono-through tetrasaccharides has been studied to assess the potential role ol thermospray ionization for microscale structural studies of saccharides and glycoconjugates, and for high sensitivity detection of liquid chromatographic effluents. Using NH4CO2H as eluant for reversed-phase HPLC, abundant MNH4+ions are formed from monosaccharides and mono-and per-methylated saccharides, and are suitable for monitoring sub-nanogram constituents in HPLC effluents. Detection of 100 pg (0.5 pmole) of 1-0-methylhexopyranosides with signal/noise >10 is demonstrated.

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Combined liquid chromatography-mass spectrometry for microscale structural studies of carbohydrates

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