Combined HPLC-thermospray mass spectrometry of mono-and disaccharides, 1-0-methylglycosides and O-permethyl mono-through tetrasaccharides has been studied to assess the potential role ol thermospray ionization for microscale structural studies of saccharides and glycoconjugates, and for high sensitivity detection of liquid chromatographic effluents. Using NH4CO2H as eluant for reversed-phase HPLC, abundant MNH4+ions are formed from monosaccharides and mono-and per-methylated saccharides, and are suitable for monitoring sub-nanogram constituents in HPLC effluents. Detection of 100 pg (0.5 pmole) of 1-0-methylhexopyranosides with signal/noise >10 is demonstrated.
|Number of pages||13|
|State||Published - Jan 1986|