Combined gas chromatography-mass spectrometry of brain polyphosphoinositides

Theodore J. Cicero, William R. Sherman

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13 Scopus citations

Abstract

The quantitative assay of tissue polyphosphoinositides has generally been accomplished by first deacylating the extracted lipids (Dittmer and Douglas, 1969) and then separating the products on an ion-exchange column. With this method, relatively large tissue samples are required, the procedure is cumbersome and the assay method, phosphorus determination, is not specific to the phosphoinositides. Thus, there has been a need for a more reliable and sensitive assay for tissue polyphosphoinositides. In this paper, we report a method for measuring and identifying MPI1 1 Abbreviations used MPI, DPI and TPI: mono-, di- and triphosphoinositide respectively; DAMPI, DADPI and DATPI, the deacylated lipids; TMS, trimethylsilyl., DPI and TPI in rat brain extracts by combined gas chromatography-mass spectrometry.

Original languageEnglish
Pages (from-to)428-433
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume42
Issue number3
DOIs
StatePublished - Feb 5 1971

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