TY - JOUR
T1 - Combined gas chromatography-mass spectrometry of brain polyphosphoinositides
AU - Cicero, Theodore J.
AU - Sherman, William R.
N1 - Funding Information:
T. J. C. wss supported by NIMR grants 07081 and 13,002. W. R. S. was supported by NIR research grant NB-05159 and Developmant Award -21,863. The mess spectrometer wss purchased by Eealth Sciences Advencemnt ksard 5 SO4 FwD61l5 and is supported by l-Sol-CAlO92601. We wish to acknowledge the help of Mr. William H. Xolland in obtaining the mess spectra.
PY - 1971/2/5
Y1 - 1971/2/5
N2 - The quantitative assay of tissue polyphosphoinositides has generally been accomplished by first deacylating the extracted lipids (Dittmer and Douglas, 1969) and then separating the products on an ion-exchange column. With this method, relatively large tissue samples are required, the procedure is cumbersome and the assay method, phosphorus determination, is not specific to the phosphoinositides. Thus, there has been a need for a more reliable and sensitive assay for tissue polyphosphoinositides. In this paper, we report a method for measuring and identifying MPI1 1 Abbreviations used MPI, DPI and TPI: mono-, di- and triphosphoinositide respectively; DAMPI, DADPI and DATPI, the deacylated lipids; TMS, trimethylsilyl., DPI and TPI in rat brain extracts by combined gas chromatography-mass spectrometry.
AB - The quantitative assay of tissue polyphosphoinositides has generally been accomplished by first deacylating the extracted lipids (Dittmer and Douglas, 1969) and then separating the products on an ion-exchange column. With this method, relatively large tissue samples are required, the procedure is cumbersome and the assay method, phosphorus determination, is not specific to the phosphoinositides. Thus, there has been a need for a more reliable and sensitive assay for tissue polyphosphoinositides. In this paper, we report a method for measuring and identifying MPI1 1 Abbreviations used MPI, DPI and TPI: mono-, di- and triphosphoinositide respectively; DAMPI, DADPI and DATPI, the deacylated lipids; TMS, trimethylsilyl., DPI and TPI in rat brain extracts by combined gas chromatography-mass spectrometry.
UR - http://www.scopus.com/inward/record.url?scp=0015211179&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(71)90388-3
DO - 10.1016/0006-291X(71)90388-3
M3 - Article
C2 - 5542891
AN - SCOPUS:0015211179
VL - 42
SP - 428
EP - 433
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -