Collision-induced dissociations of deprotonated dipeptide methyl esters containing H, alkyl or benzyl α side-chains

Adrian M. Bradford; Russell J. Waugh; John H. Bowie; David L. Vollmer; Michael L. Gross

doi:10.1016/0168-1176(94)04021-4

Collision-induced dissociations of deprotonated dipeptide methyl esters containing H, alkyl or benzyl α side-chains

Adrian M. Bradford, Russell J. Waugh, John H. Bowie, David L. Vollmer, Michael L. Gross

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The mass spectra of (M - H)^- ions derived from dipeptide methyl esters are not as useful from an analytical viewpoint as those of the underivatised peptides in that they lack the characteristic backbone cleavage which provides the primary sequencing data. Instead, major fragmentation occurs through the ester group, viz. competitive losses of MeOH and HCO₂Me are noted. Specific fragmentations differentiate between dipeptide methyl esters containing C- and N-terminal Phe. Deuterium labelling and product ion studies have aided this investigation.

Original language	English
Pages (from-to)	143-153
Number of pages	11
Journal	International Journal of Mass Spectrometry and Ion Processes
Volume	136
Issue number	2-3
DOIs	https://doi.org/10.1016/0168-1176(94)04021-4
State	Published - Sep 22 1994

Keywords

Collision-induced dissociations
Dipeptide methyl esters

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10.1016/0168-1176(94)04021-4

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@article{997200b18b584bd3a85eb1bb9861f16c,

title = "Collision-induced dissociations of deprotonated dipeptide methyl esters containing H, alkyl or benzyl α side-chains",

abstract = "The mass spectra of (M - H)- ions derived from dipeptide methyl esters are not as useful from an analytical viewpoint as those of the underivatised peptides in that they lack the characteristic backbone cleavage which provides the primary sequencing data. Instead, major fragmentation occurs through the ester group, viz. competitive losses of MeOH and HCO2Me are noted. Specific fragmentations differentiate between dipeptide methyl esters containing C- and N-terminal Phe. Deuterium labelling and product ion studies have aided this investigation.",

keywords = "Collision-induced dissociations, Dipeptide methyl esters",

author = "Bradford, {Adrian M.} and Waugh, {Russell J.} and Bowie, {John H.} and Vollmer, {David L.} and Gross, {Michael L.}",

note = "Funding Information: This work was carried out with the aid of grantsf rom the AustralianR esearchC ommit-tee and the National Science Foundation (Grant No. NSF CHE 9017250).",

year = "1994",

month = sep,

day = "22",

doi = "10.1016/0168-1176(94)04021-4",

language = "English",

volume = "136",

pages = "143--153",

journal = "International Journal of Mass Spectrometry and Ion Processes",

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TY - JOUR

T1 - Collision-induced dissociations of deprotonated dipeptide methyl esters containing H, alkyl or benzyl α side-chains

AU - Bradford, Adrian M.

AU - Waugh, Russell J.

AU - Bowie, John H.

AU - Vollmer, David L.

AU - Gross, Michael L.

N1 - Funding Information: This work was carried out with the aid of grantsf rom the AustralianR esearchC ommit-tee and the National Science Foundation (Grant No. NSF CHE 9017250).

PY - 1994/9/22

Y1 - 1994/9/22

N2 - The mass spectra of (M - H)- ions derived from dipeptide methyl esters are not as useful from an analytical viewpoint as those of the underivatised peptides in that they lack the characteristic backbone cleavage which provides the primary sequencing data. Instead, major fragmentation occurs through the ester group, viz. competitive losses of MeOH and HCO2Me are noted. Specific fragmentations differentiate between dipeptide methyl esters containing C- and N-terminal Phe. Deuterium labelling and product ion studies have aided this investigation.

AB - The mass spectra of (M - H)- ions derived from dipeptide methyl esters are not as useful from an analytical viewpoint as those of the underivatised peptides in that they lack the characteristic backbone cleavage which provides the primary sequencing data. Instead, major fragmentation occurs through the ester group, viz. competitive losses of MeOH and HCO2Me are noted. Specific fragmentations differentiate between dipeptide methyl esters containing C- and N-terminal Phe. Deuterium labelling and product ion studies have aided this investigation.

KW - Collision-induced dissociations

KW - Dipeptide methyl esters

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U2 - 10.1016/0168-1176(94)04021-4

DO - 10.1016/0168-1176(94)04021-4

M3 - Article

AN - SCOPUS:0000835721

SN - 0168-1176

VL - 136

SP - 143

EP - 153

JO - International Journal of Mass Spectrometry and Ion Processes

JF - International Journal of Mass Spectrometry and Ion Processes

IS - 2-3

ER -

Collision-induced dissociations of deprotonated dipeptide methyl esters containing H, alkyl or benzyl α side-chains

Abstract

Keywords

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