Coenzyme Q10 and lipid-related gene induction in HeLa cells

Constantine Gorelick; Melissa Lopez-Jones; Gary L. Goldberg; Seymour L. Romney; Dineo Khabele

doi:10.1016/j.ajog.2004.01.076

Coenzyme Q10 and lipid-related gene induction in HeLa cells

Constantine Gorelick, Melissa Lopez-Jones, Gary L. Goldberg, Seymour L. Romney, Dineo Khabele

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

Original language	English
Pages (from-to)	1432-1434
Number of pages	3
Journal	American journal of obstetrics and gynecology
Volume	190
Issue number	5
DOIs	https://doi.org/10.1016/j.ajog.2004.01.076
State	Published - May 2004

Keywords

Coenzyme Q10
Lipid response
Microarray

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10.1016/j.ajog.2004.01.076

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title = "Coenzyme Q10 and lipid-related gene induction in HeLa cells",

abstract = "Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.",

keywords = "Coenzyme Q10, Lipid response, Microarray",

author = "Constantine Gorelick and Melissa Lopez-Jones and Goldberg, {Gary L.} and Romney, {Seymour L.} and Dineo Khabele",

note = "Funding Information: Partially supported by P30-13330 from the National Cancer Institute. ",

year = "2004",

month = may,

doi = "10.1016/j.ajog.2004.01.076",

language = "English",

volume = "190",

pages = "1432--1434",

journal = "American Journal of Obstetrics and Gynecology",

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TY - JOUR

T1 - Coenzyme Q10 and lipid-related gene induction in HeLa cells

AU - Gorelick, Constantine

AU - Lopez-Jones, Melissa

AU - Goldberg, Gary L.

AU - Romney, Seymour L.

AU - Khabele, Dineo

N1 - Funding Information: Partially supported by P30-13330 from the National Cancer Institute.

PY - 2004/5

Y1 - 2004/5

N2 - Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

AB - Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer. Study design: We investigated the cellular and molecular effects of 30 μmol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression. Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining. Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

KW - Coenzyme Q10

KW - Lipid response

KW - Microarray

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Coenzyme Q10 and lipid-related gene induction in HeLa cells

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