CoA protects against the deleterious effects of caloric overload in Drosophila1

Laura Palanker Musselman, Jill L. Fink, Thomas J. Baranski

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

We developed a Drosophila model of T2D in which high sugar (HS) feeding leads to insulin resistance. In this model, adipose TG storage is protective against fatty acid toxicity and diabetes. Initial biochemical and gene expression studies suggested that deficiency in CoA might underlie reduced TG synthesis in animals during chronic HS feeding. Focusing on the Drosophila fat body (FB), which is specialized for TG storage and lipolysis, we undertook a series of experiments to test the hypothesis that CoA could protect against the deleterious effects of caloric overload. Quantitative metabolomics revealed a reduction in substrate availability for CoA synthesis in the face of an HS diet. Further reducing CoA synthetic capacity by expressing FB-specific RNAi targeting pantothenate kinase ( PK or fumble ) or phosphopantothenoylcysteine synthase ( PPCS ) exacerbated HS-diet-induced accumulation of FFAs . Dietary supplementation with pantothenic acid (vitamin B5, a precursor of CoA) was able to ameliorate HS-diet-induced FFA accumulation and hyperglycemia while increasing TG synthesis. Taken together, our data support a model where free CoA is required to support fatty acid esterification and to protect against the toxicity of HS diets.

Original languageEnglish
Pages (from-to)380-387
Number of pages8
JournalJournal of lipid research
Volume57
Issue number3
DOIs
StatePublished - Mar 2016

Keywords

  • Coenzyme A
  • Diabetes
  • Fatty acid/metabolism
  • Lipotoxicity
  • Nutrition
  • Obesity
  • Triglycerides

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