@inbook{6232dd26806446c09360a580dc05c94b,
title = "Co-immunoprecipitation as a Useful Tool for Detection of G Protein-Coupled Receptor Oligomers",
abstract = " Allosteric interactions between transmembrane G protein-coupled receptors (GPCRs) could lead to conformational changes and therefore oligomer function diversity increase. GPCR complexes are composed by homo- or heteroreceptors that can further assemble into receptor mosaic. These allosteric interactions could play a major role in brain regulation and plasticity. Alteration in GPCR neuromodulation may be involved in depression, schizophrenia, and addiction. Several studies reported that activation of the D 4 dopaminergic receptor blocks many of the molecular, cellular, and behavioral effects produced by morphine. The existence of a MOR/D 4 R allosteric interaction through orthosteric agonist might lead to a secure therapeutic use of morphine. Here we describe the co-immunoprecipitation technique to study direct GPCR interaction in cell culture.",
keywords = "Addiction, Co-immunoprecipitation, Dopamine 4 receptor, G protein-coupled receptors, Morphine, μ-opioid receptor",
author = "Kirill Shumilov and Alejandra Valderrama-Carvajal and Mar{\'i}a Garc{\'i}a-Bonilla and Alicia Rivera",
note = "Publisher Copyright: {\textcopyright} 2019, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2019",
doi = "10.1007/978-1-4939-8985-0_9",
language = "English",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "109--122",
booktitle = "Neuromethods",
}