Co-immunoprecipitation as a Useful Tool for Detection of G Protein-Coupled Receptor Oligomers

Kirill Shumilov, Alejandra Valderrama-Carvajal, María García-Bonilla, Alicia Rivera

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Allosteric interactions between transmembrane G protein-coupled receptors (GPCRs) could lead to conformational changes and therefore oligomer function diversity increase. GPCR complexes are composed by homo- or heteroreceptors that can further assemble into receptor mosaic. These allosteric interactions could play a major role in brain regulation and plasticity. Alteration in GPCR neuromodulation may be involved in depression, schizophrenia, and addiction. Several studies reported that activation of the D 4 dopaminergic receptor blocks many of the molecular, cellular, and behavioral effects produced by morphine. The existence of a MOR/D 4 R allosteric interaction through orthosteric agonist might lead to a secure therapeutic use of morphine. Here we describe the co-immunoprecipitation technique to study direct GPCR interaction in cell culture.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages109-122
Number of pages14
DOIs
StatePublished - 2019

Publication series

NameNeuromethods
Volume144
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Addiction
  • Co-immunoprecipitation
  • Dopamine 4 receptor
  • G protein-coupled receptors
  • Morphine
  • μ-opioid receptor

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