Clostridium butyricum MIYAIRI 588 improves high-fat diet-induced non-alcoholic fatty liver disease in rats

Makoto Seo, Ikuo Inoue, Mamoru Tanaka, Noriko Matsuda, Takanari Nakano, Takuya Awata, Shigehiro Katayama, David H. Alpers, Tsugikazu Komoda

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Background/Aims: Non-alcoholic fatty liver disease (NAFLD) has become a common liver disease, as its prevalence has increased markedly in recent decades. The aim of the present study was to examine the improving effect of Clostridium butyricum MIYAIRI 588 (CBM588), a probiotic in clinical use for antibiotic-associated diarrhea, against high-fat diet (HFD)-induced fatty liver in rats. Methods: After feeding HFD or HFD coated with CBM588 (HFD-CBM) for 12 weeks, we evaluated the hepatic mRNA levels related to lipid metabolism, and then assessed the hepatic protein levels of several transcription factors regulating these lipogenic gene expressions. Results: The HFD-CBM group had decreased accumulation of lipid droplets in the liver compared with the HFD group. The HFD-CBM group had significantly decreased diacylglycerol acyltransferase (DGAT) 2 mRNA in the liver compared with the HFD group, whereas DGAT1 mRNA did not change between the HFD group and the HFD-CBM group. Moreover, the HFD-CBM group had significantly increased hepatic mRNA regulating cholesterol catabolism enzymes and excretion transporters. Correspondingly, the HFD-CBM588 groups had increased hepatic protein levels of peroxisome proliferator-activated receptor α/γ and liver X receptor α compared with the HFD group. The HFD-CBM group had accelerated excretion of total bile acid and non-esterified fatty acid in the feces. Conclusions: CBM588 intake may have novel potential for improving NAFLD.

Original languageEnglish
Pages (from-to)3534-3544
Number of pages11
JournalDigestive diseases and sciences
Volume58
Issue number12
DOIs
StatePublished - Dec 2013

Keywords

  • CBM588
  • Diacylglycerol acyltransferase
  • Hepatic lipid droplets
  • Liver X receptor
  • Non-alcoholic fatty liver disease
  • Peroxisome proliferator-activated receptors

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