TY - JOUR
T1 - Cloning of ligand-specific cell lines via gene transfer
T2 - Identification of a D2 dopamine receptor subtype
AU - Todd, R. D.
AU - Khurana, T. S.
AU - Sajovic, P.
AU - Stone, K. R.
AU - O'Malley, K. L.
PY - 1989
Y1 - 1989
N2 - Using rat genomic DNA, we have established a transfected mouse fibroblast cell line that expresses a spiperone binding site with the pharmacological characteristics of a D2 dopamine receptor. The expressed D2 receptors are the product of a gene that is distinct from that reported by Bunzow et al. [Bunzow, J.R., Van Tol, H.H.M., Granoly, D.K., Albert, P., Salon, J., Christie, M., Machida, C.A., Neve, K.A. and Civelli, O. (1988) Nature (London) 336, 783-787]. Flow cytometry with the Ca2+-sensitive dye indo-1 demonstrated that activation of the expressed D2 sites resulted in increases in intracellular calcium that were dependent on the influx of external Ca2+. These general cloning procedures should be applicable to the production of cell lines expressing a variety of genes for which only functional assays are available.
AB - Using rat genomic DNA, we have established a transfected mouse fibroblast cell line that expresses a spiperone binding site with the pharmacological characteristics of a D2 dopamine receptor. The expressed D2 receptors are the product of a gene that is distinct from that reported by Bunzow et al. [Bunzow, J.R., Van Tol, H.H.M., Granoly, D.K., Albert, P., Salon, J., Christie, M., Machida, C.A., Neve, K.A. and Civelli, O. (1988) Nature (London) 336, 783-787]. Flow cytometry with the Ca2+-sensitive dye indo-1 demonstrated that activation of the expressed D2 sites resulted in increases in intracellular calcium that were dependent on the influx of external Ca2+. These general cloning procedures should be applicable to the production of cell lines expressing a variety of genes for which only functional assays are available.
KW - Calcium-channel activation
KW - DNA transfection
UR - http://www.scopus.com/inward/record.url?scp=0024841891&partnerID=8YFLogxK
U2 - 10.1073/pnas.86.24.10134
DO - 10.1073/pnas.86.24.10134
M3 - Article
C2 - 2532359
AN - SCOPUS:0024841891
VL - 86
SP - 10134
EP - 10138
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 24
ER -