Cloning of ligand-specific cell lines via gene transfer: Identification of a D2 dopamine receptor subtype

R. D. Todd, T. S. Khurana, P. Sajovic, K. R. Stone, K. L. O'Malley

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Using rat genomic DNA, we have established a transfected mouse fibroblast cell line that expresses a spiperone binding site with the pharmacological characteristics of a D2 dopamine receptor. The expressed D2 receptors are the product of a gene that is distinct from that reported by Bunzow et al. [Bunzow, J.R., Van Tol, H.H.M., Granoly, D.K., Albert, P., Salon, J., Christie, M., Machida, C.A., Neve, K.A. and Civelli, O. (1988) Nature (London) 336, 783-787]. Flow cytometry with the Ca2+-sensitive dye indo-1 demonstrated that activation of the expressed D2 sites resulted in increases in intracellular calcium that were dependent on the influx of external Ca2+. These general cloning procedures should be applicable to the production of cell lines expressing a variety of genes for which only functional assays are available.

Original languageEnglish
Pages (from-to)10134-10138
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number24
DOIs
StatePublished - 1989

Keywords

  • Calcium-channel activation
  • DNA transfection

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