Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases 1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes

Michael A. Becker, Steven A. Heidler, Graeme I. Bell, Susumu Seino, Michelle M. Le Beau, Carol A. Westbrook, Wilma Neuman, Larry J. Shapiro, T. K. Mohandas, Blake J. Roessler, Thomas D. Palella

    Research output: Contribution to journalArticle

    33 Scopus citations

    Abstract

    Cloned cDNAs representing the entire, homologous (80%) translated sequences of human phosphoribosylpyrophosphate synthetase (PRS) 1 and PRS 2 cDNAs were utilized as probes to localize the corresponding human PRPS1 and PRPS2 genes, previously reported to be X chromosome linked. PRPS1 and PRPS2 loci mapped to the intervals Xq22-q24 and Xp22.2-p22.3, respectively, using a combination of in situ chromosomal hybridization and human × rodent somatic cell panel genomic DNA hybridization analyses. A PRPS1-related gene or pseudogene (PRPS1L2) was also identified using in situ chromosomal hybridization at 9q33-q34. Human HPRT and PRPS1 loci are not closely linked. Despite marked cDNA and deduced amino acid sequence homology, human PRS 1 and PRS 2 isoforms are encoded by genes widely separated on the X chromosome.

    Original languageEnglish
    Pages (from-to)555-561
    Number of pages7
    JournalGenomics
    Volume8
    Issue number3
    DOIs
    StatePublished - Nov 1990

    Fingerprint Dive into the research topics of 'Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases 1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes'. Together they form a unique fingerprint.

  • Cite this

    Becker, M. A., Heidler, S. A., Bell, G. I., Seino, S., Le Beau, M. M., Westbrook, C. A., Neuman, W., Shapiro, L. J., Mohandas, T. K., Roessler, B. J., & Palella, T. D. (1990). Cloning of cDNAs for human phosphoribosylpyrophosphate synthetases 1 and 2 and X chromosome localization of PRPS1 and PRPS2 genes. Genomics, 8(3), 555-561. https://doi.org/10.1016/0888-7543(90)90043-T