Cloning and tissue distribution of two new potassium channel α-subunits from rat brain

Martin Stocker, Daniel Kerschensteiner

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The expressed sequence tag (EST) database is a valuable tool to identify functionally related clones, when sequence similarity is so low that standard library screening methods cannot be successfully applied. Comparing conserved protein sequences of cloned voltage-gated potassium channels led to the identification and cloning of a new putative potassium channel α-subunit from rat brain, Kv9.1. A homologue of this α-subunit was also found in human brain tissue. A second α-subunit, Kv9.3, most probably belonging to the same subfamily, was also isolated and sequenced. Tissue distribution and analysis of genomic DNA were performed for both channels. rKv9.1 is expressed exclusively in the central nervous system, whereas rKv9.3 shows a widespread tissue distribution. No currents could be measured in X. oocytes upon injection of rKv9.1 or rKv9.3 cRNA.

Original languageEnglish
Pages (from-to)927-934
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume248
Issue number3
DOIs
StatePublished - Jul 30 1998

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