TY - JOUR
T1 - Cloning and localization of Rab3 isoforms in bovine, rat, and human parathyroid glands
AU - Huang, Zhengmin
AU - Ritter, Cindy
AU - Brown, Alex
AU - Finch, Jane
AU - Abu-Amer, Yousef
AU - Ross, Patrick
AU - Slatopolsky, Eduardo
N1 - Funding Information:
This research work was supported in part by National Institute of Diabetes and Digestive and Kidney Diseases grants DK-09976, DK-30178 and DK-07126. We thank Dr. Adriana Dusso for helpful discussions during the course of this study and critical reviewing of this manuscript. We also would like to thank Marilyn Aach Levy for her technical expertise on immunogold electron microscopic work.
PY - 1999/2/24
Y1 - 1999/2/24
N2 - Rab3 proteins are small GTP-binding proteins known to play a role in regulated exocytosis processes. This study examines the expression of Rab3 mRNA and protein in bovine, rat and human parathyroid glands. mRNAs of several Rab3 isoforms were detected in bovine (Rab3A, Rab3B and Rab3C) and rat (Rab3A, Rab3B and Rab3D) parathyroid glands by RT-PCR and sequencing. Rab3A protein was detected in the cytosolic extract from bovine parathyroid gland by Western blotting using a monoclonal antibody for Rab3A. Rab3A protein was localized to parathyroid hormone-containing chief cells by immunohistochemical staining. Subcellular localization of Rab3A protein by immunogold electron microscopy revealed that the majority of Rab3A protein was not associated with dense-core vesicles, but localized in the cytosol of the chief cells. Altogether, our results demonstrate that Rab3 isoforms are expressed in parathyroid chief cells, suggesting that they may play a role in regulated exocytosis in these cells.
AB - Rab3 proteins are small GTP-binding proteins known to play a role in regulated exocytosis processes. This study examines the expression of Rab3 mRNA and protein in bovine, rat and human parathyroid glands. mRNAs of several Rab3 isoforms were detected in bovine (Rab3A, Rab3B and Rab3C) and rat (Rab3A, Rab3B and Rab3D) parathyroid glands by RT-PCR and sequencing. Rab3A protein was detected in the cytosolic extract from bovine parathyroid gland by Western blotting using a monoclonal antibody for Rab3A. Rab3A protein was localized to parathyroid hormone-containing chief cells by immunohistochemical staining. Subcellular localization of Rab3A protein by immunogold electron microscopy revealed that the majority of Rab3A protein was not associated with dense-core vesicles, but localized in the cytosol of the chief cells. Altogether, our results demonstrate that Rab3 isoforms are expressed in parathyroid chief cells, suggesting that they may play a role in regulated exocytosis in these cells.
UR - http://www.scopus.com/inward/record.url?scp=0033599316&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0226
DO - 10.1006/bbrc.1999.0226
M3 - Article
C2 - 10049764
AN - SCOPUS:0033599316
SN - 0006-291X
VL - 255
SP - 645
EP - 651
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -