Cloning and expression of an inwardly rectifying ATP-regulated potassium channel

Kevin Ho; Colin G. Nichols; W. Jonathan Lederer; Jonathan Lytton; Peter M. Vassilev; Marie V. Kanazirska; Steven C. Hebert

doi:10.1038/362031a0

Cloning and expression of an inwardly rectifying ATP-regulated potassium channel

Kevin Ho, Colin G. Nichols, W. Jonathan Lederer, Jonathan Lytton, Peter M. Vassilev, Marie V. Kanazirska, Steven C. Hebert

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Abstract

A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney. The predicted 45K protein, which features two potential membrane-spanning helices and a proposed ATP-binding domain, represents a major departure from the basic structural design characteristic of voltage-gated and second messenger-gated ion channels. But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.

Original language	English
Pages (from-to)	31-38
Number of pages	8
Journal	Nature
Volume	362
Issue number	6415
DOIs	https://doi.org/10.1038/362031a0
State	Published - 1993

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title = "Cloning and expression of an inwardly rectifying ATP-regulated potassium channel",

abstract = "A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney. The predicted 45K protein, which features two potential membrane-spanning helices and a proposed ATP-binding domain, represents a major departure from the basic structural design characteristic of voltage-gated and second messenger-gated ion channels. But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.",

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AU - Ho, Kevin

AU - Nichols, Colin G.

AU - Lederer, W. Jonathan

AU - Lytton, Jonathan

AU - Vassilev, Peter M.

AU - Kanazirska, Marie V.

AU - Hebert, Steven C.

PY - 1993

Y1 - 1993

N2 - A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney. The predicted 45K protein, which features two potential membrane-spanning helices and a proposed ATP-binding domain, represents a major departure from the basic structural design characteristic of voltage-gated and second messenger-gated ion channels. But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.

AB - A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney. The predicted 45K protein, which features two potential membrane-spanning helices and a proposed ATP-binding domain, represents a major departure from the basic structural design characteristic of voltage-gated and second messenger-gated ion channels. But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.

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Cloning and expression of an inwardly rectifying ATP-regulated potassium channel

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