Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins

Hongming Ma, Kangsen Mai, Zhiguo Liufu, Wei Xu

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

An actin gene (CfACT1) was cloned by using RT-PCR, 3' and 5'RACE from hemocytes of the sea scallop Chlamys farreri. The full length of the transcript is 1 535 bp, which contains a long 3' un-translated region of 436bp and 59bp of a 5' un-translated sequence. The open reading frame encodes a polypeptide of 376 amino acids. Sequence comparisons indicated that CfACT1 is more closely related to vertebrate cytoplasmic actins than muscle types. Phylogenetic analysis showed that molluscan actins could be generally divided into two categories: muscle and cytoplasmic, although both are similar to vertebrate cytoplasmic actins. It was also inferred that different isotypes existed in muscle or cytoplasma in mollusks. The genomic sequence of CfACT1 was cloned and sequenced. Only one intron was detected: it was located between codons 42 and 43 and different from vertebrate actin genes.

Original languageEnglish
Pages (from-to)304-309
Number of pages6
JournalChinese Journal of Oceanology and Limnology
Volume25
Issue number3
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

Keywords

  • Chlamys farreri
  • actin
  • clone
  • intron
  • phylogenetic analysis

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